Overview

  • Product name

    Human TRAIL ELISA Kit (CD253)
    See all TRAIL kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    A 40 1500pg/ml 82.9 5.5%
    B 33 116.3pg/ml 8.7 7.4%
    Inter-assay
    Sample n Mean SD CV%
    A 12 1619pg/ml 62.8 3.8%
    B 12 162.8pg/ml 13.1 8%
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 64 pg/ml
  • Range

    93.75 pg/ml - 3000 pg/ml
  • Assay time

    3h 45m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Human TRAIL (CD253) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab46074) is designed for the quantitative measurement of TRAIL in cell culture supernatants, buffered solutions or Human serum, plasma, and other body fluids.


    A monoclonal antibody specific for TRAIL has been coated onto the wells of the microtiter strips provided. Samples, including standards of known TRAIL concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for TRAIL are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of TRAIL present in the samples.


    This kit will recognize both endogenous and recombinant Human TRAIL.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests 2 x 96 tests
    10X Standard Diluent Buffer Black 1 x 25ml 1 x 25ml
    200X Wash Buffer White 1 x 10ml 2 x 10ml
    Biotinylated Antibody Diluent Red 1 x 7ml 1 x 13ml
    Biotinylated anti-TRAIL Red 1 x 400µl 2 x 400µl
    Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml
    Control Silver 2 vials 4 vials
    HRP Diluent Red 1 x 23ml 1 x 23ml
    Plastic Plate Covers 2 units 4 units
    Stop Reagent Black 1 x 11ml 2 x 11ml
    Streptavidin-HRP 2 x 5µl 4 x 5µl
    TRAIL Microplate (12 x 8 well strips) 1 unit 2 units
    TRAIL Standard (Lyophilized) Yellow 2 vials 4 vials
  • Research areas

  • Relevance

    TRAIL is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. It preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. TRAIL binds to several members of TNF receptor superfamily including TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly also to TNFRSF11B/OPG. The activity of TRAIL may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis. The binding of TRAIL to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3.
  • Cellular localization

    Cell Membrane; single-pass type II membrane protein.
  • Alternative names

    • Apo 2 ligand
    • APO2L
    • CD253
    • Protein TRAIL
    • TL2
    • TNF related apoptosis inducing ligand
    • Tumor necrosis factor ligand superfamily member 10
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab46074 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • TRAIL measured in undiluted cell culture supernatants from control (-) or stimulated ((P/I); 48 hours with 50 ng x mL-1 PMA (ab120297) and 1 uM ionomycin (ab120116)) Jurkat cells (duplicates +/- SD).

  • TRAIL measured in undiluted biological fluids (duplicates +/- SD).

  • Representative Standard Curve using ab46074

Protocols

References

This product has been referenced in:

  • Kurt IC  et al. KDM2B, an H3K36-specific demethylase, regulates apoptotic response of GBM cells to TRAIL. Cell Death Dis 8:e2897 (2017). Read more (PubMed: 28661478) »
  • Wang J  et al. Silencing the epigenetic silencer KDM4A for TRAIL and DR5 simultaneous induction and antitumor therapy. Cell Death Differ 23:1886-1896 (2016). Human . Read more (PubMed: 27612013) »
See all 3 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Question
Answer

Thank you for your call today and for letting us know about the trouble with ab46074.
As we discussed, I'm sending a free of charge replacement kit, which should arrive tomorrow on the order ***.
Please keep me updated about the performance of this new kit, and let me know if there is anything else that we can do for you.

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Question
Answer

At this time we haveno information regarding the cross reactivity between our human TRAIL ELISA (ab46074)and the murine protein.

Therefore, I can offer a discount off a future purchase if you buy ab46074 now, test it inmouse and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free ELISA kit OR 20% off a protein or primary antibody product.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab46074 in mouse. I will then send a discount code. This code must be issued before purchasing ab46074 so please wait for my reply before ordering.

2. Purchase ab46074 either by phone, fax, or online (www.abcam.com).

3. Test it in mouse.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for 1 ELISA kit OR 20% off a protein or primary antibody product and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab46074 turns out to be unsuitable for mouse you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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