Overview

  • Product name

    Human TRAIL R2 ELISA Kit
    See all DR5 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    HepG2 cells 9 1.9%
    Inter-assay
    Sample n Mean SD CV%
    HepG2 cells 3 3.5%
  • Sample type

    Serum, Cell culture extracts, Tissue Extracts, EDTA Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    10.1 pg/ml
  • Range

    78.13 pg/ml - 5000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 91 90% - 93%
    Cell culture extracts 100 99% - 100%
    Tissue Extracts 105 104% - 105%
    EDTA Plasma 81 80% - 82%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    TRAIL R2 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of TRAIL R2 protein in human serum, plasma (EDTA), and cell and tissue extracts.


     


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    TRAIL R2, also known as Tumor necrosis factor receptor superfamily member 10B, Death Receptor 5, TNF-related apoptosis-inducing ligand receptor 2 (TRAIL) and CD262, is a type 1 membrane bound, cell surface receptor for the ligand TNFSF10, or TRAIL, and is encoded by the gene TNFRSF10B. TRAIL R2 contains a 155- amino acid extracellular region, a 21- amino acid transmembrane section, and a 209- amino acid cytoplasmic region. The extracellular domain contains three TNFR-Cys regions and the cytoplasmic domain contains a death domain. TRAIL R2 is activated upon TRAIL binding, which transduces an apoptosis signal. The adapter molecule FADD recruits caspase-8 to the activated receptor, which results in the death-inducing signaling complex (DISC) that initiates a cascade of caspases. The antibodies in this product were raised to the extracellular domain of TRAIL R2.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human TRAIL R2 Capture Antibody 1 x 600µl
    10X Human TRAIL R2 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent 4BR 1 x 6ml
    Human TRAIL R2 Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 12ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Function

    Receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B. Essential for ER stress-induced apoptosis.
  • Tissue specificity

    Widely expressed in adult and fetal tissues; very highly expressed in tumor cell lines such as HeLaS3, K-562, HL-60, SW480, A-549 and G-361; highly expressed in heart, peripheral blood lymphocytes, liver, pancreas, spleen, thymus, prostate, ovary, uterus, placenta, testis, esophagus, stomach and throughout the intestinal tract; not detectable in brain.
  • Involvement in disease

    Squamous cell carcinoma of the head and neck
  • Sequence similarities

    Contains 1 death domain.
    Contains 3 TNFR-Cys repeats.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Alternative names

    • Fas like protein
    • Apoptosis inducing protein TRICK2A/2B
    • Apoptosis inducing receptor TRAIL R2
    • CD262
    • CD262 antigen
    • Cytotoxic TRAIL receptor 2
    • Death domain containing receptor for TRAIL/Apo 2L
    • Death receptor 5
    • DR5
    • KILLER
    • KILLER/DR5
    • OTTHUMP00000123492
    • OTTHUMP00000123493
    • p53 regulated DNA damage inducible cell death receptor(killer)
    • TNF related apoptosis inducing ligand receptor 2
    • TNF-related apoptosis-inducing ligand receptor 2
    • TNFRSF10B
    • TR10B_HUMAN
    • TRAIL R2
    • TRAIL receptor 2
    • TRAIL-R2
    • TRAILR2
    • TRICK2
    • TRICK2A
    • TRICK2B
    • TRICKB
    • Tumor necrosis factor receptor like protein ZTNFR9
    • Tumor necrosis factor receptor superfamily member 10B
    • Tumor necrosis factor receptor superfamily, member 10b
    • ZTNFR9
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab233614 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • The TRAIL R2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • The TRAIL R2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of TRAIL R2 were measured in duplicates, interpolated from the TRAIL R2 standard curves and corrected for sample dilution. Undiluted samples are as follows: A549 extract 250 µg/mL, MDA-MB-231 extract 62.5 µg/mL, HepG2 extract 62.5 µg/mL, and HT1080 extract 31.25 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

  • The concentrations of TRAIL R2 were measured in duplicate, interpolated from the TRAIL R2 standard curve and corrected for sample dilution. Undiluted samples are both 500 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

  • The concentrations of TRAIL R2 were measured in duplicate and interpolated from the TRAIL R2 standard curve and corrected for sample dilution. Undiluted samples are follows: serum 50% and plasma (EDTA) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Protocols

References

ab233614 has not yet been referenced specifically in any publications.

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