Human Transferrin ELISA Kit (ab108902)
- Datasheet
- Specific References (2)
- Protocols
Overview
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Product nameHuman Transferrin ELISA Kit
See all Transferrin kits -
Detection methodColorimetric
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Precision
Intra-assay Sample n Mean SD CV% Overall 3.7% Inter-assay Sample n Mean SD CV% Overall 9.9% -
Sample typeCell culture supernatant, Saliva, Milk
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Assay typeSandwich (quantitative)
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Sensitivity= 1 ng/ml
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Range1.563 ng/ml - 100 ng/ml
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Recovery
97 %
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Assay time4h 00m
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Assay durationMultiple steps standard assay
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Species reactivityReacts with: Human
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Product overview
Abcam’s Tranferrin Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Transferrin in urine, milk saliva, CSF and cell culture supernatants.
A Tranferrin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Tranferrin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Tranferrin captured in plate.
Get results in 90 minutes with Human Transferrin ELISA Kit (ab187391) from our SimpleStep ELISA® range.
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Tested applicationsSuitable for: Sandwich ELISAmore details
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PlatformMicroplate
Properties
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Storage instructionsStore at -20°C. Please refer to protocols.
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Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Transferrin Antibody 1 x 120µl Chromogen Substrate 1 x 8ml Sealing Tapes 3 units Stop Solution 1 x 12ml Transferrin Microplate (12 x 8 well strips) 1 unit Transferrin Standard 1 vial -
Research areas
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FunctionTransferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.
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Tissue specificityExpressed by the liver and secreted in plasma.
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Involvement in diseaseAtransferrinemia
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Sequence similaritiesBelongs to the transferrin family.
Contains 2 transferrin-like domains. -
Cellular localizationSecreted.
- Information by UniProt
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Alternative names
- ATRAF
- Atransferrinemia
- Beta-1 metal-binding globulin
see all -
Database links
- Entrez Gene: 7018 Human
- Omim: 209300 Human
- SwissProt: P02787 Human
- Unigene: 518267 Human
Associated products
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SimpleStep ELISA kits
Applications
Our Abpromise guarantee covers the use of ab108902 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
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Typical Standard CurveRepresentative Standard Curve using ab108902.
Protocols
References
This product has been referenced in:
- Mehta SR et al. Cerebrospinal fluid cell-free mitochondrial DNA is associated with HIV replication, iron transport, and mild HIV-associated neurocognitive impairment. J Neuroinflammation 14:72 (2017). ELISA ; Human . Read more (PubMed: 28359324) »
- Patton SM et al. Cerebrospinal fluid (CSF) biomarkers of iron status are associated with CSF viral load, antiretroviral therapy, and demographic factors in HIV-infected adults. Fluids Barriers CNS 14:11 (2017). ELISA ; Human . Read more (PubMed: 28427421) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"