Overview

  • Product name
    Human Transferrin ELISA Kit
    See all Transferrin kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 8 2.6%
    Inter-assay
    Sample n Mean SD CV%
    Overall 3 4.2%
  • Sample type
    Cell culture supernatant, Serum, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    292 pg/ml
  • Range
    312.5 pg/ml - 20000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 103 101% - 106%
    Cell culture media 88 87% - 89%
    Heparin Plasma 104 101% - 107%
    EDTA Plasma 103 100% - 104%
    Citrate Plasma 101 100% - 102%

  • Assay time
    1h 30m
  • Assay duration
    One step assay
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat, Rabbit, Goat, Guinea pig, Hamster, Cow, Dog, Pig
  • Product overview

    Transferrin in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Transferrin protein in human serum, plasma and cell culture supernatant samples.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • Notes

    Transferrins are iron binding transport proteins which can bind two Fe3+ ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Transferrin Capture Antibody 1 x 600µl
    10X Transferrin Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    ab221824 - Antibody Diluent 4BI 1 x 6ml
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
    Transferrin Human Lyophilized Purified Protein 2 vials
  • Research areas
  • Function
    Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.
  • Tissue specificity
    Expressed by the liver and secreted in plasma.
  • Involvement in disease
    Atransferrinemia
  • Sequence similarities
    Belongs to the transferrin family.
    Contains 2 transferrin-like domains.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Alternative names
    • ATRAF
    • Atransferrinemia
    • Beta-1 metal-binding globulin
    • PRO1400
    • Serotransferrin
    • Siderophilin
    • TF
    • Transferrin
    • TRFE_HUMAN
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab187391 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Example of Transferrin standard curve. Background-subtracted data values (mean +/- SD) are graphed.

  • Titration of Human serum within the working range of the assay. Background-subtracted data values (mean +/- SD, n = 3) are graphed.

  • FObserved Transferrin levels in pooled donor normal Human plasma. Mean Human Transferrin values fall within expected normal reference ranges (WHO).

  • Observed Transferrin levels in individual donor normal Human serum (n=10). Mean Human Transferrin is 1.05 mg/mL and values fall within expected normal reference ranges (WHO).

Protocols

References

This product has been referenced in:
  • Anantpadma M  et al. Large Scale Screening and Identification of Novel Ebolavirus and Marburgvirus Entry Inhibitors. Antimicrob Agents Chemother N/A:N/A (2016). Sandwich ELISA ; Human . Read more (PubMed: 27161622) »

See 1 Publication for this product

Customer reviews and Q&As

Yes, the capture and detector antibody recognize human Transferrin.

1) The antibodies in this kit have been raised against native transferrin and unfortunately we do not know precise epitopes. A monoclonal capture antibody and a polyclonal detector antibody is provided. Therefore we do not know if the mutations gene...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up