Overview

  • Product name

    Human Transferrin Receptor ELISA Kit
    See all Transferrin Receptor kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 8 2%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 1.3%
  • Sample type

    Saliva, Urine, Serum, Cell culture extracts, Hep Plasma, EDTA Plasma, Cit plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    1.26 pg/ml
  • Range

    23.4 pg/ml - 1500 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Saliva 94 92% - 96%
    Urine 89 89% - 89%
    Serum 102 96% - 108%
    Cell culture extracts 107 106% - 107%
    Hep Plasma 106 100% - 113%
    EDTA Plasma 105 104% - 107%
    Cit plasma 97 93% - 101%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
    Does not react with: Cow
  • Product overview

    Transferrin receptor in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of transferrin receptor protein in human serum, plasmas, saliva, urine, and cell extracts. 


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Sensitivity:


    Samples in 1X Cell Extraction Buffer PTR: 1.26 pg/mL


    Samples in Sample Diluent NS: 3.25 pg/mL

  • Notes

    Transferrin receptor is a 760 amino acid, transmembrane glycoprotein composed of two disulfide-linked monomers encoded by the gene TFRC. Also known as CD71 and TfR1, transferrin receptor is necessary to regulate iron transport into cells from transferrin by receptor mediated endocytosis. The soluble form of transferrin receptor is due to the proteolysis of Arg-100 in the extracellular domain. Measurements of soluble transferrin receptor may be an indication of iron deficiency due to chronic diseases.   

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human Transferrin Receptor Capture Antibody 1 x 600µl
    10X Human Transferrin Receptor Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent 5BI 1 x 6ml
    Human Transferrin Receptor Lyophilized Recombinant Protein 2 units
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Function

    Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
    (Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.
  • Involvement in disease

    Immunodeficiency 46
  • Sequence similarities

    Belongs to the peptidase M28 family. M28B subfamily.
    Contains 1 PA (protease associated) domain.
  • Post-translational
    modifications

    N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
    Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
    Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.
  • Cellular localization

    Secreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Alternative names

    • CD 71
    • CD71
    • CD71 antigen
    • IMD46
    • OTTHUMP00000208523
    • OTTHUMP00000208524
    • OTTHUMP00000208525
    • p90
    • sTfR
    • T9
    • TfR
    • TFR 1
    • TfR1
    • TFR1_HUMAN
    • TFRC
    • TR
    • Transferrin receptor (p90 CD71)
    • Transferrin receptor protein 1, serum form
    • Trfr
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab217780 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of transferrin receptor were measured in duplicate, interpolated from the Transferrin Receptor standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:2000, plasma (citrate) 1:1000, plasma (EDTA) 1:2000, and plasma (heparin) 1:2000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Transferrin Receptor concentration was determined to be 1648 ng/mL in serum, 1490 ng/mL in plasma (citrate), 1957 ng/mL in plasma (EDTA) and 1692 ng/mL in plasma (heparin).

  • The concentrations of Transferrin Receptor were measured in duplicate, interpolated from the Transferrin Receptor standard curves and corrected for sample dilution. Undiluted samples are as follows: saliva 100%, urine 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Transferrin Receptor concentration was determined to be 358 pg/mL in saliva.

  • The concentrations of transferrin receptor were measured in duplicate and interpolated from the transferrin receptor standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Transferrin Receptor concentration was determined to be 513 ng/mL in A549 cell extract.

  • Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). They were converted to nmol/L from pg/mL by dividing by 76,000 to align with commonly reported units. The Transferrin Receptor concentration was determined to be 21.9 nmol/L with a range of 10.8 – 40 nmol/L.

Protocols

References

ab217780 has not yet been referenced specifically in any publications.

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