Overview

  • Product name

    Human TREM1 ELISA Kit
    See all TREM1 kits
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 70 pg/ml
  • Range

    0.069 ng/ml - 50 ng/ml
  • Recovery

    > 100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 124.4 106% - 134%
    Serum 125.5 91% - 145%
    Plasma 116 94% - 115%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s TREM1 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TREM1 in serum, plasma and cell culture supernatants. (Human TREM1 concentration is pretty low in normal serum, it may not be detected in this assay).


    This assay employs an antibody specific for Human TREM1 coated on a 96-well plate. Standards and samples are pipetted into the wells and TREM1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TREM1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TREM1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    Get higher sensitivity in only 90 minutes with Human soluble TREM1 ELISA Kit (ab185987) from our SimpleStep ELISA® range.

  • Notes

    Optimization may be required with urine samples.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    500X HRP-Streptavidin Concentrate 1 x 200µl
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Human TREM1 2 vials
    Recombinant Human TREM1 Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
    TREM1 Microplate (12 x 8 wells) 1 unit
  • Research areas

  • Relevance

    TREM1 is also known as triggering receptor expressed on myeloid cells 1, and triggering receptor expressed on monocytes 1. TREM1 is a Type I membrane protein that contains an immunoglobulin-like V-type domain. An alternatively spliced protein variant may be secreted. TREM1 is highly expressed on peripheral blood myeloid cells (particularly mature monocytes and granulocytes); TREM1 expression can be further upregulated by bacteria, fungi and lipopolysaccharide. TREM1 has been shown to interact with the adaptor protein DAP12 to stimulate neutrophil and monocyte-mediated inflammatory responses through the triggering and release of pro-inflammatory cytokines and chemokines. TREM1 is thought to amplify inflammatory responses to fungal and bacterial infections and potentiate septic shock.
  • Cellular localization

    Secreted and Cell membrane.
  • Alternative names

    • CD354
    • TREM 1
    • Triggering receptor expressed on monocytes 1
    • Triggering receptor expressed on myeloid cells 1
    • Triggering receptor TREM 1
    • Triggering receptor TREM1
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab100659 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab100659

  • Representative standard curve using ab100659

Protocols

References

ab100659 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Answer

Thank you for your email.

I can confirm that you will find all of the available QC data about serum and plasma near the end of the protocol booklet including spiking&recovery and linearity tests.

Furthermore I like to confirm that the levels measured testing normal human serum and plasma were undetectable with this kit. Therefore we would recommend this kit for diseased or treated serum or plasma samples with expected elevated levels of TREM-1 than normal.

I hope this information is helpful to you. Please do not hesitate to contact us again if you have further questions.

Read More

Answer

Thank you for contacting us.

The kit ab100659 TREM1 Human ELISA Kit is suitable to quantify the soluble form of human TREM-1.

Unfortunately, the concentrations used to coat the microplate and the one for the biotynilated antibody are considered to be proprietary.

The antibody’s immunogen sequence is also proprietary information. The exact epitope sequence recognized by the antibodies is unknown, we only know the antibody pair is binding somewhere on the extracellular domain (aa 21-205).

In regards to clonality, the capture antibody is monoclonal and the detection antibody is polyclonal.

I hope this helps. Please let me know if you have any questions or comments.

Read More

Answer

The report I received from the laboratory tells us that it will be impossible to predict if this ELISA kit can detect the splice variant you are interested in, given the information we have about the antibodies.

From the UniProt page for human TREM1:

Isoform 2 (identifier: Q9NP99-2)

Also known as: TREM-1sv; sTREM1;

The sequence of this isoform differs from the canonical sequence as follows:
138-150: SGTPGSNENSTQN RCSTLSFSWLVDS
151-234: Missing.

http://www.uniprot.org/uniprot/Q9NP99

Here is what I received from the lab:

The immunogen used to raise the capture antibody in this kit was a recombinant human TREM-1, aa 21-205 (accession # Q9NP99). The immunogen for the detection antibody was a recombinant human TREM-1, aa 1-200 (same accession #). Because epitope mapping has not been performed, the exact epitopes that the antibodies are binding to is not known. We just know that the capture antibody is binding somewhere between aa 21-205 and the detection antibody is binding somewhere between aa 1-200.

Read More

Answer

Thank you for your inquiry.

It is recommended to plot the standard curve using a log-log model as stated in the Protocol Booklet. Log-log (common log) is recommended as this model fits most data sets well. However, other graphing models can certainly be used like linear regression, 4-parameter, 2-order polynomial, etc. I would advise you to plot the data in a few models and then use whichever fits best.

I am afraid that we are not familiar with Biorad Microplate Manager 5.2 software and there are pre-settings in your machine and/or software that only the maker would know. Therefore, we unable to comment on the program's optimal settings for use with this kit. Please kindly redirect your questions to the software manufacturer.


I am sure the software manufacturer would be able to help you further.

Read More

Answer

Thank you for contacting us and your interest in our products.
The TREM1 Human ELISA Kit (ab100659) uses the diluent A and B in sample preparation of serum/plasma and cell culture medium/urine respectively.
The Assay Diluent A is optimized for dilution of serum and plasma samples. Assay Diluent B is for cell culture supernates, urine, and/or other sample types. The diluent separately represent the matrix of their respective sample types and should be used accordingly for optimum performance.
We are unfortunately not able to share the composition of the diluent, but the main difference is that a small amount of sodium azide is in Assay Diluent A, but not in Assay Diluent B. Sodium azide can inhibit the HRP-streptavidin function. Therefore, Assay Diluent A should not be used for dilution of the detection antibody or the HRP-streptavidin reagents.
I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up