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    human-trem2-elisa-kit-ab224881.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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RecombinantSimpleStep

Human TREM2 ELISA Kit (ab224881)

  • Datasheet
  • SDS
  • Protocol Booklet
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Other - Human TREM2 ELISA Kit (ab224881)
  • Example of Human TREM2 standard curve in Sample Diluent NS.
  • Example of Human TREM2 standard curve in Sample Diluent NS.
  • Example of Human TREM2 standard curve in 1X Cell Extraction Buffer PTR.
  • Example of Human TREM2 standard curve in 1X Cell Extraction Buffer PTR.
  • Interpolated concentrations of native TREM2 in Human serum and plasma samples.
  • Serum from ten individual healthy Human female donors was measured in duplicate.
  • Interpolated concentrations of native TREM2 in Human cerebrospinal fluid and THP-1 cell culture supernatant samples.
  • Interpolated concentrations of native TREM2 in human THP-1 cell extract and human placenta tissue extract.
  • Linearity of dilution.
  • Linearity of dilution.
  • Linearity of dilution.
  • Sandwich ELISA - Human TREM2 ELISA Kit (ab224881)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 10.5 pg/ml
  • Range: 78.1 pg/ml - 5000 pg/ml
  • Sample type: Cell culture extracts, Cell culture media, Cerebral Spinal Fluid, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

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Overview

  • Product name

    Human TREM2 ELISA Kit
    See all TREM2 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 5 6.3%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 4.9%
  • Sample type

    Serum, Cell culture extracts, Tissue Extracts, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma, Cerebral Spinal Fluid
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    10.5 pg/ml
  • Range

    78.1 pg/ml - 5000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 93 85% - 104%
    Cell culture extracts 109 100% - 117%
    Tissue Extracts 99 95% - 101%
    Cell culture media 91 86% - 94%
    Hep Plasma 103 97% - 111%
    EDTA Plasma 101 93% - 105%
    Cit plasma 95 90% - 103%
    Cerebral Spinal Fluid 111 109% - 113%
  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
    Does not react with: Cow
  • Product overview

    Human TREM2 ELISA Kit (ab224881) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TREM2 protein in cell culture extracts, cell culture media, cerebral spinal fluid, cit plasma, edta plasma, hep plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human TREM2 with 10.5 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.


    ASSAY SPECIFICITY This kit recognizes both native and recombinant Human TREM2 protein in serum, plasma, cerebrospinal fluid and cell culture supernatant, cell and tissue extract samples only.


    SPECIES REACTIVITY This kit recognizes Human TREM2 protein.
    Other species reactivity was determined by measuring 25% serum samples of various species, interpolating the protein concentrations from the Human standard curve, and expressing the interpolated concentrations as a percentage of the protein concentration in Human serum assayed at the same dilution. 
    Reactivity < 3% was determined for the following species: Mouse, Rat and cow.

  • Notes

    TREM2 is immune receptor expressed on the surface of microglia.  TREM2 is proteolytically process by ADAM proteases and the soluble TREM2 is released into the extra cellular space. Elevated levels of soluble TREM2 in cerebrospinal fluid is a biomarker associated with increased risk of Alzheimer’s disease. Ab224881 TREM2 SimpleStep ELISA® is designed against the extracellular portion of TREM2.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Wash Buffer PT (ab206977) 1 x 20ml
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml
    10X Human TREM2 Capture Antibody 1 x 600µl
    10X Human TREM2 Detector Antibody 1 x 600µl
    Human TREM2 Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Neuroscience
    • Development
    • Neuroscience
    • Processes
  • Function

    May have a role in chronic inflammations and may stimulate production of constitutive rather than inflammatory chemokines and cytokines. Forms a receptor signaling complex with TYROBP and triggers activation of the immune responses in macrophages and dendritic cells.
  • Tissue specificity

    Expressed on macrophages and dendritic cells but not on granulocytes or monocytes. In the CNS strongest expression seen in the basal ganglia, corpus callosum, medulla oblongata and spinal cord.
  • Involvement in disease

    Defects in TREM2 are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL) [MIM:221770]; also known as presenile dementia with bone cysts or Nasu-Hakola disease (NHD). PLOSL is a recessively inherited disease characterized by a combination of psychotic symptoms rapidly progressing to presenile dementia and bone cysts restricted to wrists and ankles. PLOSL has a global distribution, although most of the patients have been diagnosed in Finland and Japan, with an estimated population prevalence of 2x10(-6) in the Finns.
  • Sequence similarities

    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization

    Secreted and Cell membrane.
  • Target information above from: UniProt accession Q9NZC2 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • TREM 2
    • TREM-2
    • TREM2
    • TREM2_HUMAN
    • TREM2a
    • TREM2b
    • TREM2c
    • Trggering receptor expressed on myeloid cells 2
    • Trggering receptor expressed on myeloid cells 2a
    • Triggering receptor expressed on monocytes 2
    • Triggering receptor expressed on myeloid cells 2
    see all
  • Database links

    • Entrez Gene: 54209 Human
    • Omim: 605086 Human
    • SwissProt: Q9NZC2 Human
    • Unigene: 435295 Human

    Associated products

    • Alternative Versions

      • Human TREM2 Antibody Pair - BSA and Azide free (ab244027)

    Images

    • Other - Human TREM2 ELISA Kit (ab224881)
      Other - Human TREM2 ELISA Kit (ab224881)

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

       

    • Example of Human TREM2 standard curve in Sample Diluent NS.
      Example of Human TREM2 standard curve in Sample Diluent NS.

      Background-subtracted data values (mean +/- SD) are graphed.

    • Example of Human TREM2 standard curve in Sample Diluent NS.
      Example of Human TREM2 standard curve in Sample Diluent NS.

      Example of Human TREM2 standard curve in Sample Diluent NS. The TREM2 standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

    • Example of Human TREM2 standard curve in 1X Cell Extraction Buffer PTR.
      Example of Human TREM2 standard curve in 1X Cell Extraction Buffer PTR.

      Background-subtracted data values (mean +/- SD) are graphed.

    • Example of Human TREM2 standard curve in 1X Cell Extraction Buffer PTR.
      Example of Human TREM2 standard curve in 1X Cell Extraction Buffer PTR.

      Example of Human TREM2 standard curve in 1X Cell Extraction Buffer PTR. The TREM2 standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed

    • Interpolated concentrations of native TREM2 in Human serum and plasma samples.
      Interpolated concentrations of native TREM2 in Human serum and plasma samples.

      The concentrations of TREM2 were measured in duplicates, interpolated from the TREM2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 12.5%, plasma (EDTA) 12.5%, plasma (heparin) 12.5%, and plasma (citrate) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TREM2 concentration was determined to be 23.8 ng/mL in serum, 19.0 ng/mL in plasma (EDTA), 25.3ng/mL in plasma (heparin) and 16.5 ng/mL in plasma (citrate).

    • Serum from ten individual healthy Human female donors was measured in duplicate.
      Serum from ten individual healthy Human female donors was measured in duplicate.

      Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TREM2 concentration was determined to be 30 ng/mL with a range of 13 – 66 ng/mL.

    • Interpolated concentrations of native TREM2 in Human cerebrospinal fluid and THP-1 cell culture supernatant samples.
      Interpolated concentrations of native TREM2 in Human cerebrospinal fluid and THP-1 cell culture supernatant samples.

      The concentrations of TREM2 were measured in duplicates, interpolated from the TREM2 standard curves and corrected for sample dilution. Undiluted samples are as follows: cerebrospinal fluid 25% and THP-1 cell culture supernatant 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TREM2 concentration was determined to be 19.8 ng/mL in cerebrospinal fluid, and 8.4 ng/mL in THP-1 cell culture supernatant.

    • Interpolated concentrations of native TREM2 in human THP-1 cell extract and human placenta tissue extract.
      Interpolated concentrations of native TREM2 in human THP-1 cell extract and human placenta tissue extract.

      The concentrations of TREM2 were measured in duplicate and interpolated from the TREM2 standard curve and corrected for sample dilution and extract load. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TREM2 concentration was determined to be 15.7 pg/µg in THP-1 cell extract and 0.34 pg/µg in placenta tissue extract.

    • Linearity of dilution.
      Linearity of dilution.

      Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

      Native TREM2 was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.

    • Linearity of dilution.
      Linearity of dilution.

      Native TREM2 was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.

    • Linearity of dilution.
      Linearity of dilution.

      Native TREM2 was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in 1X Cell Extraction Buffer PTR.

    • Sandwich ELISA - Human TREM2 ELISA Kit (ab224881)
      Sandwich ELISA - Human TREM2 ELISA Kit (ab224881)
      To learn more about the advantages of recombinant antibodies see here.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (6)

    Publishing research using ab224881? Please let us know so that we can cite the reference in this datasheet.

    ab224881 has been referenced in 6 publications.

    • Diaz-Lucena D  et al. TREM2 expression in the brain and biological fluids in prion diseases. Acta Neuropathol 141:841-859 (2021). PubMed: 33881612
    • Hu HY  et al. Associations of Sleep Characteristics with Cerebrospinal Fluid sTREM2 in Cognitively Normal Older Adults: the CABLE Study. Neurotox Res 39:1372-1380 (2021). PubMed: 34097185
    • Li W  et al. Increased CSF Soluble TREM2 Concentration in Patients With Neurosyphilis. Front Neurol 11:62 (2020). PubMed: 32117023
    • Ma LZ  et al. Dynamic changes of CSF sTREM2 in preclinical Alzheimer's disease: the CABLE study. Mol Neurodegener 15:25 (2020). PubMed: 32276587
    • Li W  et al. Cerebrospinal Fluid Cytokines in Patients with Neurosyphilis: The Significance of Interleukin-10 for the Disease. Biomed Res Int 2020:3812671 (2020). PubMed: 33083463
    • Hou XH  et al. Genome-wide association study identifies Alzheimer's risk variant in MS4A6A influencing cerebrospinal fluid sTREM2 levels. Neurobiol Aging 84:241.e13-241.e20 (2019). PubMed: 31204042

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