Human TREM2 ELISA Kit (ab224881)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Human TREM2 ELISA Kit
See all TREM2 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 5 6.3% Inter-assay Sample n Mean SD CV% Serum 3 4.9% -
Sample type
Serum, Cell culture extracts, Tissue Extracts, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma, Cerebral Spinal Fluid -
Assay type
Sandwich (qualitative) -
Sensitivity
10.5 pg/ml -
Range
78.1 pg/ml - 5000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 93 85% - 104% Cell culture extracts 109 100% - 117% Tissue Extracts 99 95% - 101% Cell culture media 91 86% - 94% Hep Plasma 103 97% - 111% EDTA Plasma 101 93% - 105% Cit plasma 95 90% - 103% Cerebral Spinal Fluid 111 109% - 113% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human
Does not react with: Cow -
Product overview
TREM2 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of TREM2 protein in Human serum, plasma, cell culture supernatant, cerebrospinal fluid, cell and tissue extract samples.
The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
TREM2 is immune receptor expressed on the surface of microglia. TREM2 is proteolytically process by ADAM proteases and the soluble TREM2 is released into the extra cellular space. Elevated levels of soluble TREM2 in cerebrospinal fluid is a biomarker associated with increased risk of Alzheimer’s disease. Ab224881 TREM2 SimpleStep ELISA® is designed against the extracellular portion of TREM2.
Sensitivity:
Sample diluted in Sample Diluent NS - 36 pg/mL
Sample diluted in 1X Cell Extraction Buffer PTR - 10.5 pg/mL
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml 10X Human TREM2 Capture Antibody 1 x 600µl 10X Human TREM2 Detector Antibody 1 x 600µl Human TREM2 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
May have a role in chronic inflammations and may stimulate production of constitutive rather than inflammatory chemokines and cytokines. Forms a receptor signaling complex with TYROBP and triggers activation of the immune responses in macrophages and dendritic cells. -
Tissue specificity
Expressed on macrophages and dendritic cells but not on granulocytes or monocytes. In the CNS strongest expression seen in the basal ganglia, corpus callosum, medulla oblongata and spinal cord. -
Involvement in disease
Defects in TREM2 are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL) [MIM:221770]; also known as presenile dementia with bone cysts or Nasu-Hakola disease (NHD). PLOSL is a recessively inherited disease characterized by a combination of psychotic symptoms rapidly progressing to presenile dementia and bone cysts restricted to wrists and ankles. PLOSL has a global distribution, although most of the patients have been diagnosed in Finland and Japan, with an estimated population prevalence of 2x10(-6) in the Finns. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Alternative names
- TREM 2
- TREM-2
- TREM2
see all -
Database links
- Entrez Gene: 54209 Human
- Omim: 605086 Human
- SwissProt: Q9NZC2 Human
- Unigene: 435295 Human
Associated products
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Alternative Versions
Images
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Other - Human TREM2 ELISA Kit (ab224881)SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of Human TREM2 standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Example of Human TREM2 standard curve in 1X Cell Extraction Buffer PTR.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native TREM2 in Human serum and plasma samples.The concentrations of TREM2 were measured in duplicates, interpolated from the TREM2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 12.5%, plasma (EDTA) 12.5%, plasma (heparin) 12.5%, and plasma (citrate) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TREM2 concentration was determined to be 23.8 ng/mL in serum, 19.0 ng/mL in plasma (EDTA), 25.3ng/mL in plasma (heparin) and 16.5 ng/mL in plasma (citrate).
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Serum from ten individual healthy Human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TREM2 concentration was determined to be 30 ng/mL with a range of 13 – 66 ng/mL.
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Interpolated concentrations of native TREM2 in Human cerebrospinal fluid and THP-1 cell culture supernatant samples.The concentrations of TREM2 were measured in duplicates, interpolated from the TREM2 standard curves and corrected for sample dilution. Undiluted samples are as follows: cerebrospinal fluid 25% and THP-1 cell culture supernatant 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TREM2 concentration was determined to be 19.8 ng/mL in cerebrospinal fluid, and 8.4 ng/mL in THP-1 cell culture supernatant.
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Interpolated concentrations of native TREM2 in human THP-1 cell extract and human placenta tissue extract.The concentrations of TREM2 were measured in duplicate and interpolated from the TREM2 standard curve and corrected for sample dilution and extract load. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TREM2 concentration was determined to be 15.7 pg/µg in THP-1 cell extract and 0.34 pg/µg in placenta tissue extract.
Protocols
References
This product has been referenced in:
- Hou XH et al. Genome-wide association study identifies Alzheimer's risk variant in MS4A6A influencing cerebrospinal fluid sTREM2 levels. Neurobiol Aging 84:241.e13-241.e20 (2019). Read more (PubMed: 31204042) »
