Key features and details
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Indirect
- Reacts with: Human
Product nameHuman Anti-Trichinella spiralis IgG ELISA Kit
Intra-assay Sample n Mean SD CV% Pos. Serum 8 3.9% Inter-assay Sample n Mean SD CV% Pos. Serum 4 5.2%
Sample typeSerum, Hep Plasma, Cit plasma
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Abcam’s anti-Trichinella spiralis IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Trichinella spiralis in Human serum and plasma.
A 96-well plate has been precoated with Trichinella spiralis antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Trichinella spiralis-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Trichinella spiralis IgG sample captured in plate.
Storage instructionsStore at +4°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Washing Solution White cap 1 x 50ml Cover foil 1 unit IgG Sample Diluent Colored yellow; White cap 1 x 100ml Stop Solution red cap 1 x 15ml Strip holder 1 unit TMB Substrate Solution Yellow cap 1 x 15ml Trichinella spiralis (IgG) Coated Microplate (12 x 8 wells) 12 strips of 8 wells 1 unit Trichinella spiralis IgG Cut-off Control Colored yellow; Green cap 1 x 3ml Trichinella spiralis IgG Negative Control Colored yellow; Blue cap 1 x 2ml Trichinella spiralis IgG Positive Control Colored yellow; Red cap 1 x 2ml Trichinella spiralis Protein A HRP Conjugate Colored blue; Black cap 1 x 20ml
ab108780 has not yet been referenced specifically in any publications.