Key features and details
- One-wash 90 minute protocol
- Sensitivity: 23.76 pg/ml
- Range: 54.69 pg/ml - 3500 pg/ml
- Sample type: Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Product nameHuman TrkB ELISA Kit
See all TrkB kits
Intra-assay Sample n Mean SD CV% Extract 8 0.6%
Sample typeTissue Extracts
Assay typeSandwich (quantitative)
Range54.69 pg/ml - 3500 pg/ml
Sample specific recovery Sample type Average % Range Tissue Extracts 112 108% - 118%
Assay time1h 30m
Assay durationOne step assay
Species reactivityReacts with: Human
Human TrkB ELISA Kit (ab264625) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TrkB protein in tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human TrkB with 23.76 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
TrkB, also known as NTRK2, is a tyrosine kinase receptor involved in the development of the peripheral and central nervous system. TrkB plays a role in synapse plasticity and formation, and neuron survival, proliferation and differentiation. Ligand (including BDNF and NTF4) interactions induces homodimerization, autophosphorylation and activation of TrkB. This kit targets an N-terminal fragment of the mature chain and shares approximately 90% sequence identity with mouse and rat.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
PlatformPre-coated microplate (12 x 8 well strips)
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 10X Human TrkB Capture Antibody 1 x 600µl 10X Human TrkB Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BI 1 x 6ml Human TrkB Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml
FunctionReceptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). Involved in the development and/or maintenance of the nervous system. This is a tyrosine-protein kinase receptor. Known substrates for the TRK receptors are SHC1, PI-3 kinase, and PLC-gamma-1.
Tissue specificityIsoform TrkB is widely expressed, mainly in the nervous tissue. In the CNS, expression is observed in the cerebral cortex, hippocampus, thalamus, choroid plexus, granular layer of the cerebellum, brain stem, and spinal cord. In the peripheral nervous system, it is expressed in many cranial ganglia, the ophtalmic nerve, the vestibular system, multiple facial structures, the submaxillary glands, and dorsal root ganglia. Isoform TrkB-T1 is expressed in multiple tissues, mainly in brain, pancreas, kidney and heart. Isoform TrkB-T-Shc is predominantly expressed in brain.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 2 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 LRRNT domain.
Contains 1 protein kinase domain.
- Information by UniProt
- BDNF tropomyosine receptor kinase B
- BDNF/NT 3 growth factors receptor
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
The TrkB standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed
The concentrations of TrkB were measured in duplicate and interpolated from the TrkB standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TrkB concentration was determined to be 1,934 pg/mL in brain extract.
To learn more about the advantages of recombinant antibodies see here.
ab264625 has not yet been referenced specifically in any publications.