Overview

  • Product name

    Human TSLP ELISA Kit
    See all TSLP kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 12%
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 3 pg/ml
  • Range

    3.3 pg/ml - 800 pg/ml
  • Recovery

    0 %

    Sample specific recovery
    Sample type Average % Range
    Serum 117.6 100% - 129%
    Plasma 130.7 120% - 139%
    Cell culture media 85.21 70% - 90%

  • Assay time

    5h 00m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s TSLP Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed the quantitative measurement of Human TSLP in serum, plasma and cell culture supernatants.


    This assay employs an antibody specific for Human TSLP coated on a 96-well plate. Standards and samples are pipetted into the wells and TSLP present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TSLP antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TSLP bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    Get higher sensitivity in only 90 minutes with Human TSLP ELISA Kit (ab192149) from our SimpleStep ELISA® range.

  • Notes

    Optimization may be required with urine samples.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    200X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    5X Assay Diluent D 1 x 15ml
    Detection Antibody TSLP : biotinylated anti-human TSLP 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
    TSLP Standard Human (recombinant) (lyophilized) 2 vials
    TSLP-coated Microplate 1 unit
  • Research areas

  • Relevance

    The TSLP gene encodes a hemopoietic cytokine proposed to signal through a heterodimeric receptor complex composed of the thymic stromal lymphopoietin receptor and the IL-7R alpha chain. This mainly impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes and enhances the maturation of CD11c(+) dendritic cells. The protein promotes T helper type 2 (TH2) cell responses that are associated with immunity in various inflammatory diseases, including asthma, chronic obstructive pulmonary disease and allergic inflammation. The protein is therefore considered a potential therapeutic target for the treatment of these diseases. Alternative splicing of this gene results in multiple transcript variants.
  • Cellular localization

    Secreted
  • Alternative names

    • Thymic stromal lymphopoietin
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab155444 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab155444 - Assay Diluent D.

  • Representative standard curve using ab155444 -  Assay Diluent B.

Protocols

References

This product has been referenced in:

  • Ma SL & Zhang L Elevated serum OX40L is a biomarker for identifying corticosteroid resistance in pediatric asthmatic patients. BMC Pulm Med 19:66 (2019). Read more (PubMed: 30890137) »
See 1 Publication for this product

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