Human TSLP ELISA Kit (ab155444)
Key features and details
- Sensitivity: 3 pg/ml
- Range: 3.3 pg/ml - 800 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human TSLP ELISA Kit
See all TSLP kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 12% -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 3 pg/ml -
Range
3.3 pg/ml - 800 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 117.6 100% - 129% Plasma 130.7 120% - 139% Cell culture media 85.21 70% - 90% -
Assay time
5h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s TSLP Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed the quantitative measurement of Human TSLP in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human TSLP coated on a 96-well plate. Standards and samples are pipetted into the wells and TSLP present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TSLP antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TSLP bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get higher sensitivity in only 90 minutes with Human TSLP ELISA Kit (ab192149) from our SimpleStep ELISA® range.
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Notes
Optimization may be required with urine samples.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml Detection Antibody TSLP : biotinylated anti-human TSLP 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml TSLP Standard Human (recombinant) (lyophilized) 2 vials TSLP-coated Microplate 1 unit -
Research areas
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Relevance
The TSLP gene encodes a hemopoietic cytokine proposed to signal through a heterodimeric receptor complex composed of the thymic stromal lymphopoietin receptor and the IL-7R alpha chain. This mainly impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes and enhances the maturation of CD11c(+) dendritic cells. The protein promotes T helper type 2 (TH2) cell responses that are associated with immunity in various inflammatory diseases, including asthma, chronic obstructive pulmonary disease and allergic inflammation. The protein is therefore considered a potential therapeutic target for the treatment of these diseases. Alternative splicing of this gene results in multiple transcript variants. -
Cellular localization
Secreted -
Alternative names
- Thymic stromal lymphopoietin
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Database links
- Entrez Gene: 85480 Human
- Omim: 607003 Human
- SwissProt: Q969D9 Human
Associated products
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SimpleStep ELISA kits
Images
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab155444 has been referenced in 2 publications.
- Nishimoto R et al. Human Induced Pluripotent Stem Cell-Derived Keratinocyte-Like Cells for Research on Protease-Activated Receptor 2 in Nonhistaminergic Cascades of Atopic Dermatitis . J Pharmacol Exp Ther 384:248-253 (2023). PubMed: 36351795
- Ma SL & Zhang L Elevated serum OX40L is a biomarker for identifying corticosteroid resistance in pediatric asthmatic patients. BMC Pulm Med 19:66 (2019). PubMed: 30890137