Overview

  • Product name
    Human TSLP ELISA Kit
    See all TSLP kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 8 4.4%
    Inter-assay
    Sample n Mean SD CV%
    Overall 3 5%
  • Sample type
    Cell culture supernatant, Serum
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    3 pg/ml
  • Range
    15.6 pg/ml - 1000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 88 83% - 93%
    Cell culture media 121 114% - 126%

  • Assay time
    1h 30m
  • Assay duration
    One step assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    TSLP Human protein in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of TSLP Human protein in serum and cell culture supernatant samples.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.



    Sensitivity:


    Samples diluted in Sample Diluent 50BS – 6 pg/mL
    Samples diluted in Sample Diluent NS – 3 pg/mL

  • Notes

    Thymic stromal lyphopoietin (TSLP) is a protein belonging to the IL-7 cytokine family. TSLP is released by epithelial cells and keratinocytes in response to trauma, infection or allergen. It acts upon dendritic cells to mediate TH2 mediated immune response and appears to be a key mediator in many inflammatory diseases such as: Asthma, Eczema, and Atopic Dermatitis. TSLP also acts upon mast cells promote the release of IL-5, -6, and -13 in the presence of IL-1 and TNF-α.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X TSLP Capture Antibody 1 x 600µl
    10X TSLP Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    ab221824 - Antibody Diluent 4BI 1 x 6ml
    Plate Seals 1 unit
    Sample Diluent 50BS 1 x 20ml
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
    TSLP Human Lyophilized Recombinant Protein 2 vials
  • Research areas
  • Relevance
    The TSLP gene encodes a hemopoietic cytokine proposed to signal through a heterodimeric receptor complex composed of the thymic stromal lymphopoietin receptor and the IL-7R alpha chain. This mainly impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes and enhances the maturation of CD11c(+) dendritic cells. The protein promotes T helper type 2 (TH2) cell responses that are associated with immunity in various inflammatory diseases, including asthma, chronic obstructive pulmonary disease and allergic inflammation. The protein is therefore considered a potential therapeutic target for the treatment of these diseases. Alternative splicing of this gene results in multiple transcript variants.
  • Cellular localization
    Secreted
  • Alternative names
    • Thymic stromal lymphopoietin
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab192149 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The samples and standard curve was diluted in Sample Diluent 50BS. Background-subtracted data values (mean +/- SD, n = 2) are graphed.

  • Severity of A.D. symptoms and medical histories of all donors are unknown. Samples and standard curve was diluted in Sample Diluent 50BS. Background-subtracted data values (mean +/- SD, n = 2) are graphed.

Protocols

References

ab192149 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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