Overview

  • Product name

    Human U-Plasminogen Activator ELISA Kit
    See all Urokinase kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Urine 5 1.7%
    Inter-assay
    Sample n Mean SD CV%
    Urine 3 2.6%
  • Sample type

    Cell culture supernatant, Urine, Serum, Cell culture extracts, Hep Plasma, EDTA Plasma, Cit plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    4 pg/ml
  • Range

    62.5 pg/ml - 4000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 108 96% - 113%
    Urine 106 97% - 115%
    Serum 87 85% - 90%
    Cell culture extracts 84 83% - 86%
    Hep Plasma 87 86% - 88%
    EDTA Plasma 91 88% - 93%
    Cit plasma 89 89% - 90%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
    Does not react with: Cow
  • Product overview

    U-Plasminogen Activator in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of U-Plasminogen Activator protein in Human  serum, plasma, cell culture supernatant, urine, and cell extract.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    U-Plasminogen Activator (also known as Urokinase-type plasminogen activator) is a secreted serine protease which activates plasminogen to plasmin.  Mature U-Plasminogen Activator is a disulfide linked dimer of A and B chains.  The antibodies in this kit were generated to the A chain and recognize both the A chain and the A-B dimer.


    Sensitivity:


    Samples diluted in Sample Diluent NS - 44 pg/mL


    Samples diluted in 1X Cell Extraction Buffer PTR - 4 pg/mL


     

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Applications

Our Abpromise guarantee covers the use of ab226904 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of U-Plasminogen Activator were measured in duplicates, interpolated from the U-Plasminogen Activator standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 25%, plasma (heparin) 25%, and plasma (EDTA) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean U-Plasminogen Activator concentration was determined to be 439 pg/mL in serum, 732 pg/mL in plasma (citrate), 678 pg/mL in plasma (heparin), and 871 pg/ml in plasma (EDTA).

  • The concentrations of U-Plasminogen Activator were measured in duplicates, interpolated from the U-Plasminogen Activator standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 50%, plasma (heparin) 50%, and plasma (EDTA) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

  • Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean U-Plasminogen Activator concentration was determined to be 500 pg/mL with a range of 265 – 745 pg/mL.

  • The concentrations of U-Plasminogen Activator were measured in duplicates, interpolated from the U-Plasminogen Activator standard curves and corrected for sample dilution. Undiluted samples are as follows: HepG2 supernatant 25%, PC-3 supernatant 1:250, and Urine 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean U-Plasminogen Activator concentration was determined to be 10 ng/mL in HepG2 supernatant, 865 ng/mL in PC-3 supernatant, and 73 ng/mL in Urine.

  • The concentrations of U-Plasminogen Activator were measured in duplicate and interpolated from the U-Plasminogen Activator standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean U-Plasminogen Activator concentration was determined to be 4689 pg/mL in PC-3 Cell Extract.

Protocols

References

ab226904 has not yet been referenced specifically in any publications.

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