Human uPA ELISA Kit (URK) (ab119611)
Key features and details
- Sensitivity: 5 pg/ml
- Range: 62.5 pg/ml - 4000 pg/ml
- Sample type: Cell culture supernatant, Cell Lysate, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human uPA ELISA Kit (URK)
See all Urokinase kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% 1 16 1211pg/ml 8.77 = 4.5% 2 16 583pg/ml 39.06 = 6.7% 3 16 1373pg/ml 74.14 = 5.4% Inter-assay Sample n Mean SD CV% 1 24 211pg/ml 12.87 = 6.1% 2 24 536pg/ml 44.48 = 8.3% 3 24 1493pg/ml 103.01 = 6.9% -
Sample type
Cell culture supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 5 pg/ml -
Range
62.5 pg/ml - 4000 pg/ml -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Human uPA (URK) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human uPA in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
A uPA specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for uPA is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human uPA amount of sample captured in plate.
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Notes
Plasminogen activator,urokinase (PLAU, uPA) converts plasminogen to plasmin. Plasmin is involved in processing of amyloid precursor protein and degrades secreted and aggregated amyloid-beta, a hallmark of Alzheimer disease (AD). Urokinase has a molecular mass of about 54 kD and is composed of 2 disulfide-linked chains, A and B, of molecular masses 18 kD and 33 kD, respectively. It localized on 10q24. uPA facilitates cell migration by localizing proteolisys on the cell surface and by inducing intracellular signalling pathways. In human vascular smooth muscle cell (VSMC), uPA stimulates migration via the uPA receptor (uPAR) signalling complex containing TYK2 and phosphatidylinositol 3-kinase (PI3-K).
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Antibody Diluent Buffer Green Cap 1 x 12ml Anti-Human uPA antibody Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl Biotinylated anti-Human uPA antibody 1 x 100µl Lyophilized recombinant Human uPA standard 2 x 10ng Plate Seal 4 units Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Amber Bottle 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml Wash Buffer (25X) 1 x 20ml -
Research areas
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Function
Specifically cleave the zymogen plasminogen to form the active enzyme plasmin. -
Tissue specificity
Expressed in the prostate gland and prostate cancers. -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 1 EGF-like domain.
Contains 1 kringle domain.
Contains 1 peptidase S1 domain. -
Post-translational
modificationsPhosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- ATF
- ATF uPA
- BDPLT5
see all -
Database links
- Entrez Gene: 5328 Human
- Omim: 191840 Human
- SwissProt: P00749 Human
- Unigene: 77274 Human
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab119611 has been referenced in 4 publications.
- Dzhauari S et al. Urokinase-Type Plasminogen Activator Enhances the Neuroprotective Activity of Brain-Derived Neurotrophic Factor in a Model of Intracerebral Hemorrhage. Biomedicines 10:N/A (2022). PubMed: 35740368
- Nantajit D et al. Prognostic significance of uPA and uPAR expression in patients with cervical cancer undergoing radiotherapy. Oncol Lett 21:423 (2021). PubMed: 33850564
- Peteri UK et al. Urokinase plasminogen activator mediates changes in human astrocytes modeling fragile X syndrome. Glia 69:2947-2962 (2021). PubMed: 34427356
- Karagyaur M et al. A Bicistronic Plasmid Encoding Brain-Derived Neurotrophic Factor and Urokinase Plasminogen Activator Stimulates Peripheral Nerve Regeneration After Injury. J Pharmacol Exp Ther 372:248-255 (2020). PubMed: 31888957