Overview

  • Product name

  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 5 pg/ml
  • Range

    62.5 pg/ml - 4000 pg/ml
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s Human uPA (URK) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human uPA in cell culture supernatants, serum and plasma (heparin, EDTA).

    A uPA specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for uPA is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human uPA amount of sample captured in plate.

  • Notes

    Plasminogen activator,urokinase (PLAU, uPA) converts plasminogen to plasmin. Plasmin is involved in processing of amyloid precursor protein and degrades secreted and aggregated amyloid-beta, a hallmark of Alzheimer disease (AD). Urokinase has a molecular mass of about 54 kD and is composed of 2 disulfide-linked chains, A and B, of molecular masses 18 kD and 33 kD, respectively. It localized on 10q24. uPA facilitates cell migration by localizing proteolisys on the cell surface and by inducing intracellular signalling pathways. In human vascular smooth muscle cell (VSMC), uPA stimulates migration via the uPA receptor (uPAR) signalling complex containing TYK2 and phosphatidylinositol 3-kinase (PI3-K).

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    ABC Diluent Buffer Blue Cap 1 x 12ml
    Antibody Diluent Buffer Green Cap 1 x 12ml
    Anti-Human uPA antibody Microplate (12 x 8 wells) 1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
    Biotinylated anti-Human uPA antibody 1 x 130µl
    Lyophilized recombinant Human uPA standard 2 x 10ng
    Plate Seal 4 units
    Sample Diluent Buffer Green Cap 1 x 30ml
    TMB Color Developing Agent Amber Bottle 1 x 10ml
    TMB Stop Solution Yellow Cap 1 x 10ml
  • Research areas

  • Function

    Specifically cleave the zymogen plasminogen to form the active enzyme plasmin.
  • Tissue specificity

    Expressed in the prostate gland and prostate cancers.
  • Sequence similarities

    Belongs to the peptidase S1 family.
    Contains 1 EGF-like domain.
    Contains 1 kringle domain.
    Contains 1 peptidase S1 domain.
  • Post-translational
    modifications

    Phosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • ATF
    • BDPLT5
    • Plasminogen activator urinary
    • PLAU
    • QPD
    • u PA
    • U-plasminogen activator
    • uPA
    • URK
    • UROK_HUMAN
    • Urokinase-type plasminogen activator chain B
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab119611 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab119611

Protocols

References

ab119611 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

uPA ELISA - use with citrated plasma

Good Excellent 5/5 (Ease of Use)
Abreviews
We used this kit to quantify urokinase levels in citrated plasma samples. Samples were thawed from -80degrees Celsius and diluted with the sample diluent buffer. The assay was conducted in accordance with the recommended protocol, which was clear and easy to follow throughout.

We encountered no major issues in data acquisition. The attached figure demonstrates the standard curve we obtained and the distribution of sample readings.

Abcam user community

Verified customer

Submitted Dec 12 2016

Answer

Siento no haber contestado antes, me temo que no tengo la información de todos los productos solicitados, sin embargo, puedo proporcionarte acerca de los siguientes kits:

El kit PLTP Activity Fluorometric Assay Kit (ab65386) no es apropiado para vuestro propósito puesto que reacciona con el ortólogo murino.

Para LIF Human ELISA Kit (ab100582) no se han llevado a cabo tests de reactividad cruzada, por lo que se desconoce si reconoce la proteína murina. El anticuerpo de captura es monoclonal y el de detección policlonal. Ambas proteínas comparten un 78% de homología, por lo que algo de reactividad cruzada es de esperar.

Respecto a Complement C3 Human ELISA kit y Fibronectin Human ELISA Kit:

ab108823: se ha observado una reactividad cruzada del 0.5% con ratón y del 10% con rata.

ab108822: ninguna reactividad cruzada observada.

ab108847: no se ha observado reactividad cruzada con el ortólogo murino

ab108848: reactividad del 5% con ratón y del 20% con rata.

Con Fibronectin Human ELISA kit (with color giving dyes) (ab119524) se ha encontrado cierta reactividad cruzada de acuerdo con el colaborador que nos lo suministra.

Para ab119611 y ab119612 (uPA Human ELISA kit y uPA Receptor Human ELISA Kit) no se ha encontrado reactividad con la proteína de ratón.

No tengo de momento información acerca de ab133037 pero si sigues interesado, en cuanto la tenga te contacto de nuevo.

Si necesitáis un presupuesto o más información acerca de cualquiera de las opciones ofrecidas, por favor, no dudéis en contactarnos de nuevo y estaré encantada de poder ayudar.

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