Overview

  • Product name

    Human Urokinase type Plasminogen Activator ELISA Kit
    See all Urokinase type Plasminogen Activator kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 4.9%
    Inter-assay
    Sample n Mean SD CV%
    Overall 7.2%
  • Sample type

    Cell culture supernatant, Urine, Serum, Plasma, Tissue
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    = 0.2 ng/ml
  • Range

    0.25 ng/ml - 8 ng/ml
  • Recovery

    98.5 %

  • Assay time

    4h 00m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s Urokinase type Plasminogen Activator Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Urokinase type Plasminogen Activator (uPA) in plasma, serum, urine, milk, cell culture supernatants and tissue extracts.


    A Urokinase type Plasminogen Activator specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Urokinase type Plasminogen Activator specific biotinylated detection antibody is added and then followed by washing with wash buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Urokinase type Plasminogen Activator captured in plate.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab108917 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution.

Images

  • Representative Standard Curve using ab108917.

Protocols

References

This product has been referenced in:

  • Zhou H  et al. Synergistic inhibitory effects of an engineered antibody-like molecule ATF-Fc and trastuzumab on tumor growth and invasion in a human breast cancer xenograft mouse model. Oncol Lett 14:5189-5196 (2017). Read more (PubMed: 29113154) »
  • Andersen H  et al. Diabetic nephropathy is associated with increased urine excretion of proteases plasmin, prostasin and urokinase and activation of amiloride-sensitive current in collecting duct cells. Nephrol Dial Transplant 30:781-9 (2015). Sandwich ELISA ; Human . Read more (PubMed: 25609736) »
See all 4 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer



Both ab108914 and ab108917 contain 12, 8 well strips.


You are correct that you should be able to detect tPA and uPA from cell culture supernatants or cell homogenates with both these kits.

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Question
Answer

Thank you for your email.

We generally recommend our customer using 400-500ul of lysis buffer per 5-10 mg of tissues however you may have to adjust it based upon your results.

500ul lysis buffer for 1 million cells would be ideal.

I hope this information will be helpful.

Read More

Question
Answer

Thank you for your email.

We generally recommend our customer using 400-500ul of lysis buffer per 5-10 mg of tissues however you may have to adjust it based upon your results.

500ul lysis buffer for 1 million cells would be ideal.

I hope this information will be helpful.

Read More

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