Overview

  • Product name

    Human Uteroglobin ELISA Kit
    See all Uteroglobin kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 5 2.3%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 4.6%
  • Sample type

    Cell culture supernatant, Saliva, Milk, Urine, Serum, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    1.4 pg/ml
  • Range

    6.3 pg/ml - 400 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 99 95% - 102%
    Saliva 101 94% - 110%
    Milk 107 98% - 124%
    Urine 106 94% - 119%
    Serum 97 90% - 105%
    Heparin Plasma 101 95% - 109%
    EDTA Plasma 100 88% - 111%
    Citrate Plasma 89 82% - 95%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat, Cow
  • Product overview

    Uteroglobin in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Uteroglobin protein in humanserum, plasma, cell culture supernatant, urine, saliva, and milk samples


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB Development Solution is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Uteroglobin is a small, glycosylated and dimeric protein expressed preimplantation in the uterus. Uteroglobin is a secreted protein found in blood, urine, saliva, and other bodily fluids where it is involved in immunomodulation.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human Uteroglobin Capture Antibody 1 x 600µl
    Human Uteroglobin Detector Antibody (Lyophilized) 1 vial
    Human Uteroglobin Lyophilized recombinant Protein 2 vials
    Antibody Diluent CPI 1 x 6ml
    10X Wash Buffer PT (ab206977) 1 x 20ml
    TMB Development Solution 1 x 12ml
    Stop Solution 1 x 12ml
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Plate Seals 1 unit
  • Research areas

  • Function

    Binds phosphatidylcholine, phosphatidylinositol, polychlorinated biphenyls (PCB) and weakly progesterone, potent inhibitor of phospholipase A2.
  • Tissue specificity

    Clara cells (nonciliated cells of the surface epithelium of the pulmonary airways).
  • Sequence similarities

    Belongs to the secretoglobin family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • Blastokinin
    • CC10
    • CC16
    • CCPBP
    • CCSP
    • Clara cell phospholipid binding protein
    • Clara cell phospholipid-binding protein
    • Clara cell specific 10 kD protein
    • Clara cells 10 kDa secretory protein
    • OTTHUMP00000236107
    • SCGB1A1
    • Secretoglobin family 1A member 1
    • Secretoglobin, family 1A, member 1 (uteroglobin)
    • UG
    • UGB
    • UP-1
    • UP1
    • Urinary protein 1
    • Urine protein 1
    • UTER_HUMAN
    • Uteroglobin
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab238266 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Standard Curve comparison between Human Uteroglobin SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of Uteroglobin were measured in duplicates, interpolated from the Uteroglobin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1: 100, plasma (citrate) 1: 100, plasma (heparin) 1: 100, and plasma (EDTA) 1: 100. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Uteroglobin concentration was determined to be 13.2 ng/mL in serum, 14.5 ng/mL in plasma (citrate), 10.7 ng/ml in plasma (heparin), and 11.4 ng/ml in plasma (EDTA).
  • The concentrations of Uteroglobin were measured in duplicates, interpolated from the Uteroglobin standard curves and corrected for sample dilution. Undiluted samples are as follows: urine (2.5%), saliva (1: 2000), and milk (25%). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Uteroglobin concentration was determined to be 5.4 ng/mL in urine, 279.8 ng/mL in saliva, and 419.2 pg/mL in milk.
  • Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Uteroglobin protein concentration was determined to be 14.7 ng/mL with a range of 6.0 - 30.0 ng/mL.

Protocols

References

ab238266 has not yet been referenced specifically in any publications.

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