Overview

  • Product name

    Human VAP-1 ELISA Kit
    See all VAP1 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    serum 8 4.8%
    Inter-assay
    Sample n Mean SD CV%
    serum 3 2.9%
  • Sample type

    Serum, Cell culture media, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    8.7 pg/ml
  • Range

    31.25 pg/ml - 2000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 105 99% - 109%
    Cell culture media 96 93% - 97%
    Heparin Plasma 100 91% - 106%
    EDTA Plasma 97 96% - 98%
    Citrate Plasma 105 97% - 115%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat
  • Product overview

    VAP-1 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of VAP-1 protein in human serum, plasma, and cell culture media.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB Development Solution is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    VAP-1 has a large extracellular domain, a single-pass transmembrane domain, and a short cytoplasmic tail. The leukocyte ligand for VAP-1 is currently unknown. Induction of VAP-1 has been shown at sites of inflammation, such as in inflammatory bowel diseases and chronic dermatoses, where expression of VAP-1 is clearly increased. It is constitutively expressed on hepatic endothelium playing a critical role in regulation of T-cell recirculation to the liver. Strong expression of VAP-1 on tumor endothelium distinguishes Human hepatocellular carcinomas from colorectal hepatic metastases. A circulating form of VAP-1 has been shown to be elevated in sera of patients with certain liver diseases and a correlation with the diagnosis of the patients was demonstrated. Human VAP-1 shares 83% sequence homology with mouse and rat.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human VAP-1 Capture Antibody 1 x 600µl
    10X Human VAP-1 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent 5BI 1 x 6ml
    Human VAP-1 Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Function

    Cell adhesion protein that participates in lymphocyte recirculation by mediating the binding of lymphocytes to peripheral lymph node vascular endothelial cells in an L-selectin-independent fashion. Has a monoamine oxidase activity. May play a role in adipogenesis.
  • Tissue specificity

    Strongly expressed on the high endothelial venules of peripheral lymph nodes and on hepatic endothelia. Also highly expressed in appendix, lung and small intestine. Expressed also in adipose tissue, in bone marrow, colon, heart, kidney, ovary, pancreas, placenta, prostate, skeletal muscle, spleen and testis.
  • Sequence similarities

    Belongs to the copper/topaquinone oxidase family.
  • Post-translational
    modifications

    Topaquinone (TPQ) is generated by copper-dependent autoxidation of a specific tyrosyl residue.
    N- and O-glycosylated.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Alternative names

    • Amine oxidase, copper containing 3 (vascular adhesion protein 1)
    • AOC3
    • AOC3_HUMAN
    • Copper amine oxidase
    • HPAO
    • Membrane copper amine oxidase
    • Membrane primary amine oxidase
    • Semicarbazide sensitive amine oxidase
    • Semicarbazide-sensitive amine oxidase
    • SSAO
    • VAP-1
    • Vascular adhesion protein 1
    • VP97
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab243684 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Standard Curve comparison between human VAP-1 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

  • The VAP-1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of VAP-1 were measured in duplicates, interpolated from the VAP-1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:400, plasma (citrate) 1:200, plasma (EDTA) 1:200 and plasma (heparin) 1:400. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VAP-1 concentration was determined to be 238.1 ng/mL in serum, 183.6 ng/mL in plasma (citrate), 196.6 ng/mL in plasma (EDTA) and 279.4 ng/mL in plasma (heparin).

  • The concentrations of VAP-1 were measured in duplicates, interpolated from the VAP-1 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

  • Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VAP-1 concentration where detectable was determined to be 512.9 ng/mL with a range of 0 – 855.8 ng/mL when diluted to 0.1% serum.

     

     

  • Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VAP-1 concentration was determined to be 345.5 ng/mL with a range of 183.1 – 454.8 ng/mL.

     

     

Protocols

References

ab243684 has not yet been referenced specifically in any publications.

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