Overview

  • Product name

    Human VCAM-1 ELISA Set (without plates)
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 0.6 ng/ml
  • Range

    1.56 ng/ml - 50 ng/ml
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s VCAM-1 Human in vitro ELISA Set is designed for the quantitative measurement of VCAM-1 in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.

     

    This ELISA Set is intended to measure human VCAM-1 in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.

    The VCAM-1 ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.

     

    Recognizes both natural and recombinant human VCAM1

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Reagents

Properties

Applications

Our Abpromise guarantee covers the use of ab47355 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • VCAM-1 Standard curve ranging from 1.56 to 50 ng/mL

References

ab47355 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thanks for your enquiry.

Please find below additional info required for ab47355 and ab47361


1) PBS is prepared as followed : 0.137mM NaCl; 1.47 KH2PO4; 8.1mM Na2PO4, 2H2O

2) Samples should be diluted : 1/100 to 1/200 for ICAM-1 to be within the standard curve range (human serum normal values are 571+/-168 ng/ml). For VCAM-1, 1/50 dilution is a good suggestion (human normal range is 626+/-340 ng/ml

3) Standards should be reconstituted, homogenized gently by up and down aspiration with the pipet and immediately added to the plate.

4) The detection Antibody is stable one year after reconstitution (if preservatives added in the reconstitution buffer). The capture antibody is stable for years if kept sterile. Substrate and enzyme should be used within 18 months after reception of the kit.



Hope this helps. Let me know if you need further info.

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