Human VCAM1 ELISA Kit (ab100661)

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Overview

  • Product name

    Human VCAM1 ELISA Kit
    See all VCAM1 kits

  • Detection method

    Colorimetric

  • Sample type

    Cell culture supernatant, Serum, Plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    < 0.3 ng/ml

  • Range

    0.247 ng/ml - 60 ng/ml

  • Recovery

    95 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 98.76 85% - 104%
    Serum 94.42 83% - 103%
    Plasma 93.58 82% - 102%

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Abcam’s VCAM1 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human VCAM1 in serum, plasma and cell culture supernatants.


    This assay employs an antibody specific for Human VCAM1 coated on a 96-well plate. Standards and samples are pipetted into the wells and VCAM1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human VCAM1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of VCAM1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    Get results in 90 minutes with Human VCAM1 ELISA Kit (CD106) (ab187393) from our SimpleStep ELISA® range.

  • Notes

    Optimization may be required with urine samples.

  • Tested applications

    Suitable for: Sandwich ELISAmore details

  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab100661 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Typical standard curve
    Typical standard curve

    Representative standard curve using ab100661

  • Typical standard curve
    Typical standard curve

    Representative standard curve using ab100661

Protocols

References

This product has been referenced in:

  • Shi J  et al. Curcumin pretreatment protects against PM2.5-induced oxidized low-density lipoprotein-mediated oxidative stress and inflammation in human microvascular endothelial cells. Mol Med Rep 16:2588-2594 (2017). Read more (PubMed: 28713935) »
  • Strøm Halden TA  et al. Short-term efficacy and safety of sitagliptin treatment in long-term stable renal recipients with new-onset diabetes after transplantation. Nephrol Dial Transplant 29:926-33 (2014). Read more (PubMed: 24452849) »
See all 2 Publications for this product

Publishing research using ab100661? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Question

Can you tell me what the host species of the primary and secondary antibodies in this kit are? Also, could you tell me the clonality of the antibodies?

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Answer

Ab46075 uses two different mouse monoclonal antibodies. However, the difference in the kits is that the capture antibody for ab100661 is a monoclonal mouse and the detection antibody is a polyclonal sheep. I hope that you find this information helpful. Please feel free to contact us again should you have further questions about this antibody or any of the products in our catalogue.    

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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