Key features and details
- Sensitivity: 15.9 pg/ml
- Range: 31.25 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Other biological fluids, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Product nameHuman VEGF-165 ELISA Kit
Intra-assay Sample n Mean SD CV% Various 3.8% Inter-assay Sample n Mean SD CV% Various 7.6%
Sample typeCell culture supernatant, Serum, Plasma, Other biological fluids, Hep Plasma, EDTA Plasma, Cit plasma
Assay typeSandwich (quantitative)
Range31.25 pg/ml - 1000 pg/ml
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Human VEGF165 ELISA kit (ab273164) is for the in-vitro qualitative and quantitative determination of VEGF165 in supernatants, buffered solutions or serum and plasma samples. This assay will recognise both natural and recombinant human VEGF165.
A capture antibody highly specific for VEGF-A has been coated to the wells of the microtiter strip plate provided during manufacture. Binding of VEGF165 in samples and known standards to the capture antibodies is completed and then any excess unbound analyte is removed.
During the next incubation period the binding of the biotinylated anti-VEGF165 secondary antibody to the analyte occurs. Any excess unbound secondary antibody is then removed.
The HRP conjugate solution is then added to every well including the zero wells, following incubation excess conjugate is removed by careful washing.
A chromogen substrate is added to the wells resulting in the progressive development of a blue coloured complex with the conjugate. The colour development is then stopped by the addition of acid turning the resultant final product yellow. The intensity of the produced coloured complex is directly proportional to the concentration of VEGF165 present in the samples and standards.
The absorbance of the colour complex is measured and the generated OD values for each standard are plotted against expected concentration forming a standard curve. This standard curve can then be used to accurately determine the concentration of VEGF165 in any sample tested.
Tested applicationsSuitable for: ELISAmore details
PlatformMicroplate (12 x 8 well strips)
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests Biotinylated Antibody diluent 1 x 7ml Biotinylated Antibody VEGF-165 1 x 400µl HRP Diluent 1 x 23ml Plastic Plate Cover 2 units Standard diluent Buffer 1 x 25ml Stop Reagent 1 x 11ml Streptavidin-HRP 2 x 5µl TMB Substrate 1 x 11ml VEGF-165 Coated Microwell strips 1 unit VEGF-165 Standard 2 vials Wash Buffer 1 x 10ml
Cellular localizationVEGF 165A: Secreted. VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.
Our Abpromise guarantee covers the use of ab273164 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
ab273164 has not yet been referenced specifically in any publications.