Overview

  • Product name

    Human VEGF-D ELISA Kit
    See all VEGFD kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    VEGF-D/serum 5 2.9%
    Inter-assay
    Sample n Mean SD CV%
    VEGF-D/serum 3 2.6%
  • Sample type

    Cell culture supernatant, Serum, Heparin Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    75.7 pg/ml
  • Range

    348.2 pg/ml - 10000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 88 85% - 92%
    Cell culture media 101 98% - 106%
    Heparin Plasma 78 74% - 82%
    Citrate Plasma 88 84% - 92%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    VEGF-D in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of VEGF-D protein in humanserum, plasma, and cell culture supernatants.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB Development Solution is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    VEGF-D, also known as vascular endothelial growth factor D, belongs to the VEGF family of proteins functioning in blood vessels development during embryogenesis and during pathological conditions including tumorogenesis. VEGF-D is a secreted homodimeric growth factor active in angiogenesis, lymphangiogenesis and endothelial cell growth, stimulating their proliferation and migration. It also has effects on the permeability of blood vessels. VEGF-D exerts its function via binding and activating VEGFR-2 (KDR/FLK1) and VEGFR-3 (FLT4) receptors.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human VEGF-D Capture Antibody 1 x 600µl
    10X Human VEGF-D Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent 5BI 1 x 6ml
    Human VEGF-D Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Function

    Growth factor active in angiogenesis, lymphangiogenesis and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in the formation of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates VEGFR-2 (KDR/FLK1) and VEGFR-3 (FLT4) receptors.
  • Tissue specificity

    Highly expressed in lung, heart, small intestine and fetal lung, and at lower levels in skeletal muscle, colon, and pancreas.
  • Sequence similarities

    Belongs to the PDGF/VEGF growth factor family.
  • Post-translational
    modifications

    Undergoes a complex proteolytic maturation which generates a variety of processed secreted forms with increased activity toward VEGFR-3 and VEGFR-2. VEGF-D first form an antiparallel homodimer linked by disulfide bonds before secretion. The fully processed VEGF-D is composed mostly of two VEGF homology domains (VHDs) bound by non-covalent interactions.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • c-fos induced growth factor
    • c-fos induced growth factor (vascular endothelial growth factor D)
    • c-fos-induced growth factor
    • FIGF
    • Vascular endothelial growth factor D
    • Vascular endothelial growth factor D precursor
    • VEGF D
    • VEGF-D
    • VEGFD
    • VEGFD_HUMAN
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab233625 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of VEGF-D were measured in duplicates, interpolated from the VEGF-D standard curves and corrected for sample dilution. Undiluted samples are as follows: serum neat, plasma (citrate) neat, and plasma (heparin) neat. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VEGF-D concentration was determined to be 2,105 pg/mL in neat serum, 1,349 pg/mL in neat plasma (citrate) and 1,232 pg/mL in neat plasma (heparin). Native signal is undetectable in RPMI cell culture supernatant containing 10% bovine serum (not shown).

  • The concentrations of VEGF-D were measured in duplicates, interpolated from the VEGF-D standard curves and corrected for sample dilution. Undiluted samples are as follows: serum neat, plasma (citrate) neat, plasma (heparin) neat, and RPMI cell culture media containing 10% FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Native signal is undetectable in RPMI cell culture supernatant containing 10% bovine serum (not shown).

  • Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VEGF-D concentration was determined to be 2,193 pg/mL with a range of 956.9 – 3,270 pg/mL.

Protocols

References

ab233625 has not yet been referenced specifically in any publications.

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