• Product name
    Human VEGF ELISA Kit
    See all VEGFA kits
  • Detection method
  • Sample type
    Cell culture extracts, Tissue Extracts
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 10 pg/ml
  • Range
    8.23 pg/ml - 6000 pg/ml
  • Recovery

    96 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture extracts 96.79 90% - 107%
    Tissue Extracts 95.58 89% - 106%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s VEGF Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human VEGF in cell lysates and tissue lysates.

    This assay employs an antibody specific for Human VEGF coated on a 96-well plate. Standards and samples are pipetted into the wells and VEGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human VEGF antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of VEGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform



Our Abpromise guarantee covers the use of ab100663 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • Hu VEGF measured in cell lysates showing quantity (pg) per million cells tested

  • Hu VEGF in various fluids showing quantity (pg) per mL of tested sample

  • Standard curve : mean of duplicates (+/-SD) with background readings subtracted



This product has been referenced in:
  • Petrica L  et al. Urinary podocyte-associated mRNA levels correlate with proximal tubule dysfunction in early diabetic nephropathy of type 2 diabetes mellitus. Diabetol Metab Syndr 9:31 (2017). Read more (PubMed: 28484521) »
  • Shimizu Y  et al. A multiplex immunoassay for the non-invasive detection of bladder cancer. J Transl Med 14:31 (2016). ELISA ; Human . Read more (PubMed: 26830497) »

See all 8 Publications for this product

Customer reviews and Q&As

Please note that ab100662 and ab100663 are similar kits that both measure levels of VEGF.

However ab100662 is best used for detecting VEGF in biological fluids such as cell culture supernatant, serum and plasma while ab100663 is best sui...

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Thank you for your enquiry.

Unfortunately, we have been unable to find any references using bone tissue extracts with the VEGF ELISA kit ab100663 nor do we have tips for preparing this type of sample. You may wish to consider trying a furthe...

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Thank you for your enquiry.

I am sorry to confirm we have not tested the effects of Urea containing lysis buffer on samples tested with ab100663 VEGF Human ELISA Kit. We would therefore not be able to confirm how well this would work. Howeve...

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Thank you for contacting us.

The concentration of the HRP-streptavidin reagent is considered proprietary information by the developer of the kit. I am sorry we could not be of more help.

Please do not hesitate to contact us if you...

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I am happy to hear that the new kits are working well for you! Please let me know if there is anything else I can do to help.

Thank you for contacting Abcam regarding our VEGF Human ELISA Kits.

The main difference between ab100663 and ab100662 is the sample types that can be analyzed. Ab100663 can be used to analyze cells and tissues, while ab100662 should be ...

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Thank you for contacting us. We unfortunately are not aware about the purpose of assaying VEGF in cell supernatant or in cell/tissue culture lysates. You may need to search literature to know more about this. The following publication will help to ...

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Thank you for your inquiry and your interest in our products. ab100663 has been specially designed and optimized for cell and tissue lysate samples. If you would like to use different type of sample, I would advise consideraing the serum, plasma, a...

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I sincerely apologize for the mix-up with the kit you had ordered.  I've put through a free of charge replacement of the Item D, 5X sample diluent.  We will ship this out to you once we receive the vial from the supplying lab. Should you still be e...

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