Human VEGF ELISA Kit (ab100663)
- Datasheet
- Specific References (8)
- Protocols
Overview
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Product nameHuman VEGF ELISA Kit
See all VEGFA kits -
Detection methodColorimetric
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Sample typeCell culture extracts, Tissue Extracts
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Assay typeSandwich (quantitative)
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Sensitivity< 10 pg/ml
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Range8.23 pg/ml - 6000 pg/ml
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Recovery
96 %
Sample specific recovery Sample type Average % Range Cell culture extracts 96.79 90% - 107% Tissue Extracts 95.58 89% - 106% -
Assay durationMultiple steps standard assay
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Species reactivityReacts with: Human
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Product overview
Abcam’s VEGF Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human VEGF in cell lysates and tissue lysates.
This assay employs an antibody specific for Human VEGF coated on a 96-well plate. Standards and samples are pipetted into the wells and VEGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human VEGF antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of VEGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applicationsSuitable for: Sandwich ELISAmore details
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PlatformMicroplate
Properties
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Storage instructionsStore at -20°C. Please refer to protocols.
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Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 2X Cell Lysate Buffer 1 x 5ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml 5X Sample Diluent Buffer 1 x 10ml Biotinylated anti-Human VEGF 2 vials Recombinant Human VEGF Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml VEGF Microplate (12 x 8 wells) 1 unit -
Research areas
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FunctionGrowth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.
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Tissue specificityIsoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed.
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Involvement in diseaseDefects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1) [MIM:603933]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
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Sequence similaritiesBelongs to the PDGF/VEGF growth factor family.
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Cellular localizationSecreted. VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.
- Information by UniProt
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Alternative names
- Folliculostellate cell-derived growth factor
- Glioma-derived endothelial cell mitogen
- MGC70609
see all -
Database links
- Entrez Gene: 7422 Human
- Omim: 192240 Human
- SwissProt: P15692 Human
- Unigene: 73793 Human
Applications
Our Abpromise guarantee covers the use of ab100663 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
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Sandwich ELISA - ab100663 VEGF Human ELISA KitHu VEGF measured in cell lysates showing quantity (pg) per million cells tested
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Sandwich ELISA - ab100663 VEGF Human ELISA KitHu VEGF in various fluids showing quantity (pg) per mL of tested sample
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Sandwich ELISA - ab100663 VEGF Human ELISA KitStandard curve : mean of duplicates (+/-SD) with background readings subtracted
Protocols
References
This product has been referenced in:
- Petrica L et al. Urinary podocyte-associated mRNA levels correlate with proximal tubule dysfunction in early diabetic nephropathy of type 2 diabetes mellitus. Diabetol Metab Syndr 9:31 (2017). Read more (PubMed: 28484521) »
- Shimizu Y et al. A multiplex immunoassay for the non-invasive detection of bladder cancer. J Transl Med 14:31 (2016). ELISA ; Human . Read more (PubMed: 26830497) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"