For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    human-vegf-elisa-kit-ab100663.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Cardiovascular Angiogenesis Growth Factors VEGF VEGF
Share by email

Human VEGF ELISA Kit (ab100663)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Q&A (9)References (19)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Sandwich ELISA - ab100663 VEGF Human ELISA Kit
  • Sandwich ELISA - ab100663 VEGF Human ELISA Kit
  • Sandwich ELISA - ab100663 VEGF Human ELISA Kit

Key features and details

  • Sensitivity: 10 pg/ml
  • Range: 8.23 pg/ml - 6000 pg/ml
  • Sample type: Cell culture extracts, Tissue Extracts
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

You may also be interested in

Protein
Product image
Recombinant human VEGF189 protein (ab106307)
ELISA
Product image
Human Anti-Dengue virus IgG ELISA Kit (ab108728)
Array
Product image
Cytokine Array – Human Cytokine Antibody Array (Membrane, 80 Targets) (ab133998)

View more associated products

Overview

  • Product name

    Human VEGF ELISA Kit
    See all VEGFA kits
  • Detection method

    Colorimetric
  • Sample type

    Cell culture extracts, Tissue Extracts
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 10 pg/ml
  • Range

    8.23 pg/ml - 6000 pg/ml
  • Recovery

    96 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture extracts 96.79 90% - 107%
    Tissue Extracts 95.58 89% - 106%
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s VEGF Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human VEGF in cell lysates and tissue lysates.

    This assay employs an antibody specific for Human VEGF coated on a 96-well plate. Standards and samples are pipetted into the wells and VEGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human VEGF antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of VEGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer 1 x 25ml
    2X Cell Lysis Buffer 1 x 5ml
    300X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent 1 x 15ml
    5X Sample Diluent Buffer 1 x 10ml
    Biotinylated anti-Human VEGF 2 vials
    Recombinant Human VEGF Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
    VEGF Microplate (12 x 8 wells) 1 unit
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Growth Factors
    • VEGF
    • VEGF
    • Signal Transduction
    • Growth Factors/Hormones
    • VEGF
    • Microbiology
    • Organism
    • Virus
    • RNA Virus
    • ssRNA positive strand virus
    • SARS Coronavirus
    • Cancer
    • Growth factors
    • VEGF
    • Cancer
    • Invasion/microenvironment
    • Angiogenesis
    • Angiogenic growth factors
    • Developmental Biology
    • Organogenesis
    • Angiogenesis and vasculogenesis
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Angiogenic factors ELISA kits
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Growth factors and hormones ELISA kits
    • Metabolism
    • Pathways and Processes
    • Cofactors, Vitamins / minerals
    • Co-factors
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Neuroscience
    • Development
    • Neuroscience
    • Processes
  • Function

    Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.
  • Tissue specificity

    Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed.
  • Involvement in disease

    Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1) [MIM:603933]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
  • Sequence similarities

    Belongs to the PDGF/VEGF growth factor family.
  • Cellular localization

    Secreted. VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.
  • Target information above from: UniProt accession P15692 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • Folliculostellate cell-derived growth factor
    • Glioma-derived endothelial cell mitogen
    • MGC70609
    • MVCD1
    • vascular endothelial growth factor
    • Vascular endothelial growth factor A
    • vascular endothelial growth factor A121
    • vascular endothelial growth factor A165
    • Vascular permeability factor
    • Vegf
    • VEGF A
    • VEGF-A
    • VEGF120
    • Vegfa
    • VEGFA_HUMAN
    • VPF
    see all
  • Database links

    • Entrez Gene: 7422 Human
    • Omim: 192240 Human
    • SwissProt: P15692 Human
    • SwissProt: Q96KJ0 Human
    • Unigene: 73793 Human

    Images

    • Sandwich ELISA - ab100663 VEGF Human ELISA Kit
      Sandwich ELISA - ab100663 VEGF Human ELISA Kit

      Hu VEGF measured in cell lysates showing quantity (pg) per million cells tested

    • Sandwich ELISA - ab100663 VEGF Human ELISA Kit
      Sandwich ELISA - ab100663 VEGF Human ELISA Kit

      Hu VEGF in various fluids showing quantity (pg) per mL of tested sample

    • Sandwich ELISA - ab100663 VEGF Human ELISA Kit
      Sandwich ELISA - ab100663 VEGF Human ELISA Kit

      Standard curve : mean of duplicates (+/-SD) with background readings subtracted

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (19)

    Publishing research using ab100663? Please let us know so that we can cite the reference in this datasheet.

    ab100663 has been referenced in 19 publications.

    • Masuda C  et al. Bevacizumab suppresses the growth of established non-small-cell lung cancer brain metastases in a hematogenous brain metastasis model. Clin Exp Metastasis 37:199-207 (2020). PubMed: 31768815
    • Liu L  et al. Enhanced Effect of IL-1ß-Activated Adipose-Derived MSCs (ADMSCs) on Repair of Intestinal Ischemia-Reperfusion Injury via COX-2-PGE2 Signaling. Stem Cells Int 2020:2803747 (2020). PubMed: 32377202
    • Wan X  et al. Hypoxia-preconditioned adipose-derived stem cells combined with scaffold promote urethral reconstruction by upregulation of angiogenesis and glycolysis. Stem Cell Res Ther 11:535 (2020). PubMed: 33308306
    • Nemati S  et al. Effect Of Bevacizumab On Growth Of Human Nasal Polyposis In Vitro; An Off-Label Use Of Anti-Angiogenic Agent For Nasal Polyposis Treatment. Drug Des Devel Ther 13:3383-3389 (2019). PubMed: 31576112
    • Wang Y  et al. The positive feedback between lncRNA TNK2-AS1 and STAT3 enhances angiogenesis in non-small cell lung cancer. Biochem Biophys Res Commun 507:185-192 (2018). PubMed: 30454892
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-9 of 9 Abreviews or Q&A

    Question

    I would like to measure VEGF in urine using an ELISA. I saw that 100662 is recommended for urine samples, but can we still use 100663?? Also, could you please clarify the difference between 100662 and 100663??

    Read More

    Abcam community

    Verified customer

    Asked on May 05 2014

    Answer


    Please note that ab100662 and ab100663 are similar kits that both measure levels of VEGF.

    However ab100662 is best used for detecting VEGF in biological fluids such as cell culture supernatant, serum and plasma while ab100663 is best suited for use with cell and tissue extracts.

    Read More

    Jeremy Kasanov

    Abcam Scientific Support

    Answered on May 05 2014

    Question

    Dear Sir,
    Is it possible to use VEGF ELISA kit (ab100663) for determining VEGF in bone tissue extracts as well?

    Sincerely,

    Read More

    Abcam community

    Verified customer

    Asked on Oct 16 2012

    Answer

    Thank you for your enquiry.

    Unfortunately, we have been unable to find any references using bone tissue extracts with the VEGF ELISA kit ab100663 nor do we have tips for preparing this type of sample. You may wish to consider trying a further search for literature regarding preparation guidelines.

    I have copied below some general tips on sample preparation, some of which may be helpful even for your bone samples so I can recommend to review this. I can also suggest to ensure not to use too much reducing or denaturing reagents when preparing the samples.

    I hope this will be helpful. Please do not hesitate to let me know if you need further assistance.

    GENERAL TIPS FOR SAMPLE PREPARATION

    Note: In case follow-up experiments are needed, it is strongly recommended to sub-aliquot all samples after preparation to minimize cytokine degradation from multiple freeze-thaw cycles.

    How do I prepare conditioned media samples?

    For testing conditioned medium, it is best to prepare serum-free or low serum medium as most serum-containing media will innately contain cytokines. If testing serum-containing medium, it is recommended to also run an uncultured media blank sample to assess the baseline responses.

    1. On day 0, seed ˜1 million cells in 100 mm tissue culture plate with complete medium.*

    2. On day 3, remove medium and replace medium with 6-8 ml of serum-free or low serum containing medium (e.g. medium containing 0.2% calf serum).

    3. On day 5, collect medium into 15 ml tube. Centrifuge at 2,000 rpm in centrifuge at 4oC for 10 minutes. Save the supernatant. Transfer the supernatant into 1.5 ml Eppendorf tubes. Store supernatant at -80ºC until experiment. Most samples can be stored this way for at least a year.

    *Cell number may be lower or higher than this depending on the cell line so the optimal number will need to be determined by each customer empirically based on researched literature and knowledge of the particular samples.


    How do I prepare plasma and serum samples?

    For plasma:

    1. Collect whole blood into an EDTA, Citrate or Sodium heparin tube

    2. Centrifuge 10 minutes at 3,000 rpm

    3. Aliquot into small tubes and store at -800C until use.

    For serum:

    1. Collect whole blood into a tube without additives

    2. Keep at room temperature for 20 minutes.

    3. Centrifuge 10 minutes at 3,000 rpm.

    4. Aliquot into small tubes and store at -800C until use.



    How do I prepare urine samples?

    1. Collect urine without adding stabilizers.

    2. Centrifuge the samples hard (eg. 10,000 x g for 1 min or 5,000 x g for 2 min).

    3. Aliquot, quick freeze in dry ice/methanol bath, and store at -800C until use.


    How do I prepare cell or tissue lysate samples?

    Cell or tissue lysates can be prepared using most conventional methods, e.g. homogenization of cell or tissue in Lysis Buffer. We supply a compatible lysis buffer in many of our kits but other general low-salt (700 mM) lysis buffers can be used with the following caveats:

    1) Avoid using > 0.1% SDS or other strongly denaturing detergents. In general, non-ionic detergents such as Triton X-100 or NP-40 are best, although zwitterionic detergents such as CHAPS, or mild ionic detergents such as sodium deoxycholate will work.

    2) Use no more than 2% v/v total detergent

    3) Avoid the use of sodium azide

    4) Avoid using >10 mM reducing agents, such as dithiothreitol or mercaptoethanols

    Note: In general, any buffers used for immunoprecipitations, including RIPA buffer, should work.

    We strongly recommend adding some type of protease inhibitor cocktail to the lysis buffer prior to homogenization.

    Since susceptibility to proteolytic cleavage and the type of proteases present in the lysate vary, we do not recommend a specific product. Instead, your choice of which combination of protease inhibitors to use should be based upon a literature search for your protein(s) of interest and/or tissue or cell type. Phosphatase inhibitors may be used but are not necessary unless the antibodies used in the kit specifically recognize phosphorylated (activated) forms of the protein.

    Choices of the method for lysis and homogenization include glass-bead smash douncing, freezethaw, sonication and crushing frozen tissue with a mortar and pestle, or even a combination of these.

    There is no one best method for all sample types, but some are better than others for some sample types. Your choice of method should be made following a brief search of the literature to see how samples similar to yours have been prepared in previous investigations.


    After homogenization, centrifuge the lysates to remove cell/tissue debris (5 min @ 10,000 x g or 10min @ 5,000 x g) and save the supernatant. Unless testing fresh, lysates should be frozen as soon as possible and stored at -20oC (or -80oC, if possible). Centrifuge them again before incubating with any immunoassay. Next, determine the protein concentration of your lysates using a total protein assay not inhibited by detergents (such as the Bicinchoninic acid (BCA) assay) and normalize the volume of each sample used to deliver the same amount of total protein for each assay.

    Note: The Bradford assay is not recommended as it can be inhibited by the presence of detergents.

    Since different cells and tissues may contain different amounts of protein, as starting point, we suggest using 500 uL of lysis buffer per 1x106 cells or 10 mg tissue. You may have to adjust this based upon your results. Your target for total protein concentration of the homogenate should be at least 1,000 ug/mL, but 2,000 ug/mL or more would be better.

    Read More

    Abcam Scientific Support

    Answered on Oct 16 2012

    Question

    Dear,

    I would like to order the VEGF Human Elisa kit ab100663 and to use it on protein extracts in lysis buffer countaining 8M urea . Do you know if the elisa should work with Urea 1M after dillution in the sample Diluent Buffer.

    Thanks a lot

    Best Regards

    Read More

    Abcam community

    Verified customer

    Asked on Sep 25 2012

    Answer

    Thank you for your enquiry.

    I am sorry to confirm we have not tested the effects of Urea containing lysis buffer on samples tested with ab100663 VEGF Human ELISA Kit. We would therefore not be able to confirm how well this would work. However, it is important to note that Urea is a Chaotropic agent at 6 - 8 M, meaning that it can denature proteins. Therefore this buffer formula may adversely affect the VEGF protein, causing it not to be recognized by the ELISA.

    I am sorry we have no data to help answer your query on this occasion, however I hope the information is helpful. If you have any further questions, please do not hesitate to contact us.

    Read More

    Abcam Scientific Support

    Answered on Sep 25 2012

    Question

    What is the concentration of the streptavidin-HRP reagent provided with this kit?

    Read More

    Abcam community

    Verified customer

    Asked on Jul 02 2012

    Answer

    Thank you for contacting us.

    The concentration of the HRP-streptavidin reagent is considered proprietary information by the developer of the kit. I am sorry we could not be of more help.

    Please do not hesitate to contact us if you have any other questions.

    Read More

    Abcam Scientific Support

    Answered on Jul 02 2012

    Question

    The new kits work fine. We think we found the problem - original kit shipped to us had manual printed in 2010 that called for diluting HRP 1:6000; new kit shipped to us has manual printed in 2012 that calls for diluting HRP 1:120.

    Read More

    Abcam community

    Verified customer

    Asked on Jun 20 2012

    Answer

    I am happy to hear that the new kits are working well for you! Please let me know if there is anything else I can do to help.

    Read More

    Abcam Scientific Support

    Answered on Jun 20 2012

    Question

    I am interested in this kit (ab100663) but want to know for the cell collection what buffers are compatible and what are not. Also, what is the difference between this kit and ab100662?

    Read More

    Abcam community

    Verified customer

    Asked on Apr 20 2012

    Answer

    Thank you for contacting Abcam regarding our VEGF Human ELISA Kits.


    The main difference between ab100663 and ab100662 is the sample types that can be analyzed. Ab100663 can be used to analyze cells and tissues, while ab100662 should be used to analyze serum, plasma, cell culture supernatants, and urine.


    Please see the guidelines below for your sample preparation of cell or tissue lysates:


    The cell or tissue lysates for use with this kit can be prepared using most conventional methods, e.g. homogenization of cell or tissue in Lysis Buffer. General low-salt lysis buffers can be used with the following caveats:


    1) Avoid using SDS or other strongly denaturing detergents. In general, non-ionic detergents, such as Triton X-100 or NP-40 are best, although zwitterionic detergents, such as CHAPS, or mild ionic detergents, such as sodium deoxycholate will work.


    2) Use no more than 2% v/v total detergent


    3) Avoid the use of sodium azide


    4) Avoid reducing agents, such as dithiothreitol or mercaptoethanols


    In general, we strongly recommend that you add some type of protein inhibitor cocktail to the lysis buffer prior to homogenization. Since susceptibility to proteolytic cleavage and the type of proteases present in the lysate vary, we do not recommend a specific product. Instead, your choice of which combination of protein inhibitors to use should be based upon a literature search for your protein(s) of interest and/or tissue or cell type. Phosphatase inhibitors may be used, but are not necessary unless the antibodies used in the kit specifically recognize phosphorylated (activated) forms of the protein.


    Choices of the method for lysis and homogenization include glass-bead smash, douncing, freeze-thaw, sonication and crushing frozen tissue with a mortar and pestle, or even a combination of these. There is no one best method for all sample types, but some are better than others for some sample types. Your choice of method should be made following a brief search of the literature to see how samples similar to yours have been prepared in previous investigations.


    After homogenization, spin down the lysates to remove cell/tissue debris (5 min @ 10000 x g or 10 min @ 5000 x g) and save the supernatant. Lysates should be frozen as soon as possible, and stored at -20C (or -80C, if possible). Spin them again before incubating with the antibody array. Determine the protein concentration of your lysates (using a Bradford-Lowry-type assay) and normalize the volume of each sample used to deliver the same amount of total protein for each assay.


    Since different cells and tissues may contain different amounts of protein, as starting point, we suggest using 500 uL of lysis buffer per 1x106 cells or 10 mg tissue. You may have to adjust this based upon your results. Your target for total protein concentration of the homogenate should be at least 1000 ug/mL, but 2000 ug/mL would be better.

    Read More

    Abcam Scientific Support

    Answered on Apr 20 2012

    Question

    thank you for your mail, which, however, did not answer my question: why measuring VEGF in cell extracts instead of measuring it in supernatants? to measure also the membrane anchored VEGF? but in this case (ie, protein supposed to increase the levels of the latter forms), is it not better optimizing to see just these forms? Are there other scientific explanations? as I told you, I intend to buy a kit and, as you commercialize both types of kits, thought you would have a clear answer to help me choose what is best for my experiments. Thank you in advance. Yours sincerely,

    Read More

    Abcam community

    Verified customer

    Asked on Nov 18 2011

    Answer

    Thank you for contacting us. We unfortunately are not aware about the purpose of assaying VEGF in cell supernatant or in cell/tissue culture lysates. You may need to search literature to know more about this. The following publication will help to start. http://www.ncbi.nlm.nih.gov/pubmed/1380254 VEGF 165, 121 are the secreted protein and 189 and 206 are the membrane bound proteins so it will be interesting to know the total amount of VEGF in cell culture samples (supernatant + lysates) however it will depend on the type of experiment and results you will be expecting. Ab100662 is a best kit for detection of VEGF in cell/tissue culture lysates while ab100663 is best for VEGF detection in serum, plasma or cell culture supernatant. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Nov 18 2011

    Question

    GOod morning, I am interested in buying in the future a VEGF elisa kit. I received an email from your company, showing such a product (catalog # ab 100663) and I noticed it measures VEGF in cell/tissue extracts. as other kits use instead cell supernatants, I was wondering if you could comment on this, i.e. advantages, if the internal level reflects the secreted levels (as there are also membrane retained forms) and so on... I am working on a protein that may change VEGF levels, acting through modulation of HIF1alpha stability. Looking forward to your answerr Sincerely

    Read More

    Abcam community

    Verified customer

    Asked on Nov 16 2011

    Answer

    Thank you for your inquiry and your interest in our products. ab100663 has been specially designed and optimized for cell and tissue lysate samples. If you would like to use different type of sample, I would advise consideraing the serum, plasma, and cell culture supernatant Human VEGF ELISA Kit (ab100662) instead. If you need any further assistance in the future, please do not hesitate to contact me.

    Read More

    Abcam Scientific Support

    Answered on Nov 16 2011

    Question

    I ordered this kit and instead of receiving "Item D, 5X Sample Diluent", I received 2 vials of "1X Assay Diluent A, Item D."

    Read More

    Abcam community

    Verified customer

    Asked on Oct 19 2011

    Answer

    I sincerely apologize for the mix-up with the kit you had ordered.  I've put through a free of charge replacement of the Item D, 5X sample diluent.  We will ship this out to you once we receive the vial from the supplying lab. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research and again I appreciate your patience with this!  

    Read More

    Abcam Scientific Support

    Answered on Oct 19 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2023 Abcam plc. All rights reserved.