Overview

  • Product name
    Human VEGF R1 ELISA Kit (FLT1)
    See all VEGF Receptor 1 kits
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 30 pg/ml
  • Range
    156 pg/ml - 10000 pg/ml
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Human soluble VEGF R1 (FLT1) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human soluble VEGF R1 (FLT1) in cell culture supernatants, serum and plasma (EDTA).


    A VEGF R1 (FLT1) specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for VEGF R1 (FLT1) is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution The density of yellow coloration is directly proportional to the Human VEGF R1 (FLT1) amount of sample captured in plate.


    Get higher sensitivity in only 90 minutes with Human VEGF R1 ELISA Kit (FLT1) (ab195210) from our SimpleStep ELISA® range.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab119613 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab119613

Protocols

References

ab119613 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for your message and I'm sorry to hear you have been having some difficulties.

Optimal dilution of the sample will usually need to be optimized by the end user. In this case, I would appreciate if you could let me know what type of samples you are using, and I can find out some recommended dilutions for you for these kits.

Thank you for your time. i look forward to hearing from you.

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Answer

Thanks for your reply.

HRP conjugated secondary antibodies:
1. For ab62183 and ab32152, you can use HRP conjugated secondary antibody ab97051
https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rabbit-IgG-HL-HRP-ab97051.html

2. For ab9540, you can use HRP conjugated secondary antibody ab97023
https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-HL-HRP-ab97023.html

Here is a link to a good general protocol for preparing tissue extracts for western blotting:
https://www.abcam.com/index.html?pageconfig=resource&rid=11375

Here are links to good general protocols for preparing tissue extracts for IHC using paraffin embedded sections:

IHC paraffin staining protocol
https://www.abcam.com/index.html?pageconfig=resource&rid=11384

Immunohistochemistry (IHC-P) protocol
https://www.abcam.com/index.html?pageconfig=resource&rid=13046

Regarding your last question "is there any draw backs while using them, since they are added for long shelf life?. What is the significance of having BSA or azide free antibody?"

There are some situations where having azide or BSA (or just too much of either) can be a drawback. For example, we have conjugation kits (EasyLink conjugation kits; https://www.abcam.com/easylink) which one can use to conjugate enzymes, fluorophores, etc to antibodies. But these conjugation reactions will not work efficiently if the concentration of BSA or azide is too high. Also, azide is a problem if the antibody needs to be injected into mouse or added to cells in culture.

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Answer

Thank you for contacting us.

I am happy to confirm that this kitwill detectfulllength and soluble VEGFR1 andtherefore the coating antibody will detect both forms as well.

I hope this information is helpful to you. IPlease do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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