Overview

  • Product name
    Human VEGF R1 ELISA Kit (FLT1)
    See all VEGF Receptor 1 kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 5 6.9%
    Inter-assay
    Sample n Mean SD CV%
    Overall 3 10.3%
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    146 pg/ml
  • Range
    0.391 ng/ml - 25 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 100 86% - 107%
    Serum 109 107% - 110%
    Heparin Plasma 105 104% - 107%
    EDTA Plasma 83 81% - 84%
    Citrate Plasma 80 78% - 81%

  • Assay time
    1h 30m
  • Assay duration
    One step assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    VEGF R1 (FLT1) in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of VEGF R1 protein in Human serum, plasma and cell culture supernatant samples.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • Notes

    VEGF R1 is a tyrosine protein kinase receptor that is essential for embryonic vasculature development, angiogenesis, cell survival, cell migration, chemotaxis, cancer cell invasion, and macrophage function. VEGF R1 can homodimerize or hererodimerize with VEGF R2, and acts as a receptor for VEGFA, VEGFB, and PGF. VEGF R1 is present as a full length, membrane form and as a truncated, soluble receptor that can also sequester ligand or dimerize with full length receptor and prevent signal transduction.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab195210 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD, n = 2) are graphed.

Protocols

References

ab195210 has not yet been referenced specifically in any publications.

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