Overview

  • Product name

    Human VEGF Receptor 2 ELISA Kit
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 70 pg/ml
  • Range

    34.3 pg/ml - 25000 pg/ml
  • Recovery

    95 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 96.23 86% - 104%
    Serum 96.46 85% - 105%
    Plasma 94.78 84% - 103%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s VEGF Receptor 2 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human VEGF Receptor 2 in serum, plasma and cell culture supernatants. (Genecards: KDR)


    This assay employs an antibody specific for Human VEGF Receptor 2 coated on a 96-well plate. Standards and samples are pipetted into the wells and VEGF Receptor 2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human VEGF Receptor 2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of VEGF Receptor 2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    600X HRP-Streptavidin Concentrate 1 x 200µl
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Human VEGF Receptor 2 2 vials
    Recombinant Human VEGF Receptor 2 Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
    VEGF Receptor 2 Microplate (12 x 8 wells) 1 unit
  • Research areas

  • Relevance

    VEGF receptor 2 is a member of a receptor tyrosine kinase family whose activation plays an essential role in a large number of biological processes such as embryonic development, wound healing, cell proliferation, migration and differentiation. Like other growth factor receptors, upon ligand binding VEGF receptor 2 dimerises and is autophosphorylated on multiple tyrosine residues. These sites can be involved in the regulation of kinase activity or serve as binding sites for SH2 and phosphotyrosine binding containing signalling proteins. Phosphorylation of Tyrosines 1054 and 1059 in the activation loop is required for activation of VEGF receptor 2 and its intrinsic tyrosine kinase activity. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
  • Cellular localization

    Cell Membrane, Cytoplasmic, Nuclear and Secreted
  • Alternative names

    • CD309
    • Fetal liver kinase 1
    • FLK1
    • KDR
    • Kinase insert domain receptor
    • Kinase insert domain receptor (a type III receptor tyrosine kinase)
    • Protein tyrosine kinase receptor Flk 1
    • Tyrosine kinase growth factor receptor
    • Vascular endothelial growth factor receptor 2
    • VEGFR
    • VEGFR 2
    • VEGFR2
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100665 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab100665

  • Representative standard curve using ab100665

Protocols

References

This product has been referenced in:

  • Kinoshita Y  et al. Mead acid inhibits the growth of KPL-1 human breast cancer cells in vitro and in vivo. Oncol Rep 32:1385-94 (2014). Read more (PubMed: 25109488) »
See 1 Publication for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

The lab does test this kit with cells cultured in serum-free media, so we aren't sure how serum in the media will directly affect the results. We suggest testing a few different culturing conditions with varying serum percent levels to find the level that best allows your cells to proliferate while interfering minimally with the assay. It would be good to also test an uncultured media blank samples to assess and subtract the baseline response of the media.

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Question
Answer

Thank you for contacting us.


Our tests show that there is no cross reactivity with mouse VEGF R2 and the Human VEGF R2 ELISA.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question
Answer

Thank you for contacting Abcam regarding this.

Unfortunately because this information is considered proprietary, I am not able to share the exact contents of the standard/sample diluent other than to tell you it is an animal serum with 0.09%sodium azide as preservative. We also do not currently carry the peptide used as standard for individual purchase.

We do carry a VEGF Receptor 2 protein (Active) (ab54347) thatconsists of all 7 extracellular domains, which contain all the information necessary for high affinity ligand binding. When developing an assay of your own, I would highly recommend this product as a standard. The product page for this is:https://www.abcam.com/VEGF-Receptor-2-protein-ab54347.html

I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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