Overview

  • Product name
    Human VEGF Receptor 2 ELISA Kit
    See all VEGF Receptor 2 kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Human serum 8 2.5%
    Inter-assay
    Sample n Mean SD CV%
    Human serum 3 5.8%
  • Sample type
    Cell culture supernatant, Serum, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    10 pg/ml
  • Range
    117 pg/ml - 7500 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 97 95% - 98%
    Serum 96 94% - 97%
    Heparin Plasma 97 95% - 99%
    EDTA Plasma 95 89% - 98%
    Citrate Plasma 114 109% - 122%

  • Assay time
    1h 30m
  • Assay duration
    One step assay
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat, Cow
  • Product overview

    Human VEGFR2 SimpleStep ELISA kit (ab192148) has been re-developed with new capture and detector antibodies.  This new kit has the same name but a different product number (ab213476). We have identified new recombinant monoclonal antibodies to use in the SimpleStep ELISA platform that provide a higher sensitivity when quantification of VEGFR2 in human serum, plasma, cell culture supernatants, and cell culture extracts.


    VEGF Receptor 2 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of VEGF Receptor 2 protein in serum, plasmas, and cell culture supernatant.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Sensitivity:
    Samples in Sample Diluent NS: 41.6 pg/mL
    Samples in Sample Diluent 3.75BS: 10 pg/mL


     

  • Notes

    VEGF R2 (vascular endothelial growth factor receptor 2) is 1356 amino acids long and is a class III receptor tyrosine kinase. VEGF R2 acts as a cell surface receptor for VEGFA, VEGFC, and VEGFD, where binding of these VEGFs to VEGF R2 activates several signaling cascades, including activation of PLCG1 (leading to the activation of protein kinase C), the MAP kinase signaling pathways, and the AKT1 signaling pathway. Furthermore, VEGF R2 plays an essential role in the regulation of angiogenesis, vascular development and permeability, embryonic hematopoiesis, and reorganization of the actin cytoskeleton. Finally, VEGF R2 is a major growth factor for endothelial cells and is the main facilitator of VEGF-induced endothelial proliferation, differentiation, and migration.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Pre-coated microplate (12 x 8 well strips)

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab213476 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of VEGF Receptor 2 were measured in duplicate, interpolated from the VEGF Receptor 2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 7.5%, plasma (citrate) 7.5%, and plasma (EDTA) 7.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VEGF Receptor 2 concentration was determined to be 60 ng/mL in serum, 42 ng/mL in plasma (citrate) and 49 ng/mL in plasma (EDTA).

  • The concentrations of VEGF Receptor 2 were measured in duplicate, interpolated from the VEGF Receptor 2 standard curve and corrected for sample dilution. Undiluted samples are as follows: plasma (heparin) 12.5% and HUVEC culture supernatant 80%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VEGF Receptor 2 concentration was determined to be 44 ng/mL in plasma (heparin). Native signal of HUVEC culture supernatant at 80% was determined to be 0.4 ng/mL.

  • Serum from ten individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VEGF Receptor 2 concentration was determined to be 2.37 ng/mL with a range of 1.19 – 3.23 ng/mL.

Protocols

References

ab213476 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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