• Product name

    Human VEGF Receptor 3 ELISA Kit
    See all VEGF Receptor 3 kits
  • Detection method

  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 15 pg/ml
  • Range

    12.29 pg/ml - 3000 pg/ml
  • Recovery

    96 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 97.35 89% - 106%
    Serum 95.46 86% - 104%
    Plasma 96.47 88% - 103%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s VEGF Receptor 3 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human VEGF Receptor 3 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human VEGF Receptor 3 coated on a 96-well plate. Standards and samples are pipetted into the wells and VEGF Receptor 3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human VEGF Receptor 3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of VEGF Receptor 3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform



  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    200X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Human VEGF Receptor 3 2 vials
    Recombinant Human VEGF Receptor 3 Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
    VEGF Receptor 3 Microplate (12 x 8 wells) 1 unit
  • Research areas

  • Relevance

    Vascular endothelial growth factors (VEGFs) are a family of closely related growth factors having a conserved pattern of eight cysteine esidues and sharing common VEGF receptors. VEGFs stimulate the proliferation of endothelial cells, induce angiogenesis, and increase vascular permeability in both large and small vessels. The mitogenic activity of VEGFs appears to be mediated by specific VEGF receptors. VEGF Receptor 3 is one of the five receptor tyrosine kinases (RTKs) (VEGF Receptor 1/Flt1, VEGF Receptor 2/KDR/Flk1, VEGF Receptor 3/Flt4, tie1 and tek/tie2) whose expression is almost exclusively restricted to endothelial cells.
  • Cellular localization

    Cell membrane; Single-pass type I membrane protein.
  • Alternative names

    • FLT 4
    • FLT4
    • FLT41
    • Fms like tyrosine kinase 4
    • Fms related tyrosine kinase 4
    • LMPH1A
    • PCL
    • Tyrosine protein kinase receptor FLT4
    • Vascular endothelial growth factor receptor 3
    • VEGFR 3
    • VEGFR3
    see all
  • Database links


Our Abpromise guarantee covers the use of ab100666 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • Hu VEGF receptor 3 measured in biofluids showing quantity (pg) per mL of tested sample

  • Representative standard curve using ab100666

  • Representative standard curve using ab100666



ab100666 has not yet been referenced specifically in any publications.

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