Human Vitronectin/S-Protein Antibody Pair - BSA and Azide free (ab244120)

Overview

  • Product name

    Human Vitronectin/S-Protein Antibody Pair - BSA and Azide free
    See all Vitronectin/S-Protein kits
  • Detection method

    Colorimetric
  • Assay type

    ELISA set
  • Range

    0.06 ng/ml - 4 ng/ml
  • Species reactivity

    Reacts with: Human
  • Product overview

    The Antibody Pair can be used to quantify Human Vitronectin. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.


    For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab234577), which uses the same antibodies. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.


    To receive an electronic copy of the Certificate of Analysis, please send an email to technical support with "CoA for matched antibody pair kit" in the subject line and the desired product number and lot number in the body of the email.


    Download SDS here.


     

  • Tested applications

    Suitable for: ELISAmore details
  • Platform

    Reagents

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 10 x 96 tests
    Human Vitronectin/S-Protein Capture Antibody 1 x 100µg
    Human Vitronectin/S-Protein Detector Antibody 1 x 100µg
  • Research areas

  • Function

    Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.
    Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity.
  • Tissue specificity

    Plasma.
  • Sequence similarities

    Contains 4 hemopexin repeats.
    Contains 1 SMB (somatomedin-B) domain.
  • Domain

    The SMB domain mediates interaction with SERPINE1/PAI1. The heparin-binding domain mediates interaction with insulin.
  • Post-translational
    modifications

    Sulfated on 2 tyrosine residues.
    N- and O-glycosylated.
    Phosphorylation on Thr-69 and Thr-76 favors cell adhesion and spreading.
    It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.
    Phosphorylation sites are present in the extracellular medium.
  • Cellular localization

    Secreted, extracellular space.
  • Information by UniProt
  • Alternative names

    • Complement S Protein
    • Epibolin
    • S Protein
    • S-protein
    • Serum Spreading Factor
    • Serum-spreading factor
    • Somatomedin B
    • Somatomedin-B
    • V75
    • Vitronectin
    • Vitronectin V10 subunit
    • Vitronectin V65 subunit
    • VN
    • VNT
    • VTN
    • VTNC_HUMAN
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab244120 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

References

ab244120 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab244120.
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