Human Vitronectin/S-Protein ELISA Kit (ab234577)
- Datasheet
- References
- Protocols
Overview
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Product name
Human Vitronectin/S-Protein ELISA Kit
See all Vitronectin/S-Protein kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Rec protein 6 3% -
Sample type
Serum -
Assay type
Sandwich (quantitative) -
Sensitivity
19.9 pg/ml -
Range
0.06 ng/ml - 4 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 114.9 107% - 119.2% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Vitronectin/S-Protein in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Vitronectin protein in human serum.
The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB Development Solution is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
Vitronectin is an extracellular matrix protein that aids in proper cell adhesion through its binding to membrane integrins. It has been implicated in tumor metastasis and invasion. Vitronectin is also a key player in platelet aggregation; reduced levels of vitronectin lead to improper clot formation.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Vitronectin/S-Protein Capture Antibody 1 x 600µl 10X Human Vitronectin/S-Protein Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BI 1 x 6ml Human Vitronectin/S-Protein Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.
Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity. -
Tissue specificity
Plasma. -
Sequence similarities
Contains 4 hemopexin repeats.
Contains 1 SMB (somatomedin-B) domain. -
Domain
The SMB domain mediates interaction with SERPINE1/PAI1. The heparin-binding domain mediates interaction with insulin. -
Post-translational
modificationsSulfated on 2 tyrosine residues.
N- and O-glycosylated.
Phosphorylation on Thr-69 and Thr-76 favors cell adhesion and spreading.
It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.
Phosphorylation sites are present in the extracellular medium. -
Cellular localization
Secreted, extracellular space. - Information by UniProt
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Alternative names
- Complement S Protein
- Epibolin
- S Protein
see all -
Database links
- Entrez Gene: 7448 Human
- Omim: 193190 Human
- SwissProt: P04004 Human
- Unigene: 2257 Human
Associated products
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Alternative Versions
Applications
Our Abpromise guarantee covers the use of ab234577 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
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Other - Human Vitronectin/S-Protein ELISA Kit (ab234577)SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human Vitronectin standard curve in 1X Cell Extraction Buffer PTR.The Vitronectin standard curve was prepared as described in Section 10.
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Interpolated concentrations of native Vitronectin in human serum samples.The concentrations of Vitronectin were measured in duplicates, interpolated from the Vitronectin standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Vitronectin concentration was determined to be 234.2 µg/mL in serum.
Protocols
References
ab234577 has not yet been referenced specifically in any publications.
