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Cardiovascular Blood Serum Proteins
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RecombinantSimpleStep

Human Vitronectin/S-Protein ELISA Kit (ab234577)

  • Datasheet
  • SDS
  • Protocol Booklet
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Other - Human Vitronectin/S-Protein ELISA Kit (ab234577)
  • Example of human Vitronectin standard curve in 1X Cell Extraction Buffer PTR.
  • Interpolated concentrations of native Vitronectin in human serum samples.
  • Sandwich ELISA - Human Vitronectin/S-Protein ELISA Kit (ab234577)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 19.9 pg/ml
  • Range: 0.06 ng/ml - 4 ng/ml
  • Sample type: Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

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Protein
Recombinant Human Vitronectin/S-Protein (ab94369)

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Overview

  • Product name

    Human Vitronectin/S-Protein ELISA Kit
    See all Vitronectin/S-Protein kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Rec protein 6 3%
  • Sample type

    Serum
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    19.9 pg/ml
  • Range

    0.06 ng/ml - 4 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 114.9 107% - 119.2%
  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Human Vitronectin/S-Protein ELISA Kit (ab234577) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Vitronectin/S-Protein protein in serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Vitronectin/S-Protein with 19.9 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human Vitronectin/S-Protein Capture Antibody 1 x 600µl
    10X Human Vitronectin/S-Protein Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent 4BI 1 x 6ml
    Human Vitronectin/S-Protein Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 12ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

    • Cardiovascular
    • Blood
    • Serum Proteins
    • Immunology
    • Innate Immunity
    • Complement
    • Regulatory
  • Function

    Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.
    Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity.
  • Tissue specificity

    Plasma.
  • Sequence similarities

    Contains 4 hemopexin repeats.
    Contains 1 SMB (somatomedin-B) domain.
  • Domain

    The SMB domain mediates interaction with SERPINE1/PAI1. The heparin-binding domain mediates interaction with insulin.
  • Post-translational
    modifications

    Sulfated on 2 tyrosine residues.
    N- and O-glycosylated.
    Phosphorylation on Thr-69 and Thr-76 favors cell adhesion and spreading.
    It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.
    Phosphorylation sites are present in the extracellular medium.
  • Cellular localization

    Secreted, extracellular space.
  • Target information above from: UniProt accession P04004 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • Complement S Protein
    • Epibolin
    • S Protein
    • S-protein
    • Serum Spreading Factor
    • Serum-spreading factor
    • Somatomedin B
    • Somatomedin-B
    • V75
    • Vitronectin
    • Vitronectin V10 subunit
    • Vitronectin V65 subunit
    • VN
    • VNT
    • VTN
    • VTNC_HUMAN
    see all
  • Database links

    • Entrez Gene: 7448 Human
    • Omim: 193190 Human
    • SwissProt: P04004 Human
    • Unigene: 2257 Human

    Associated products

    • Alternative Versions

      • Human Vitronectin/S-Protein Antibody Pair - BSA and Azide free (ab244120)

    Images

    • Other - Human Vitronectin/S-Protein ELISA Kit (ab234577)
      Other - Human Vitronectin/S-Protein ELISA Kit (ab234577)

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

       

    • Example of human Vitronectin standard curve in 1X Cell Extraction Buffer PTR.
      Example of human Vitronectin standard curve in 1X Cell Extraction Buffer PTR.

      The Vitronectin standard curve was prepared as described in Section 10.

    • Interpolated concentrations of native Vitronectin in human serum samples.
      Interpolated concentrations of native Vitronectin in human serum samples.

      The concentrations of Vitronectin were measured in duplicates, interpolated from the Vitronectin standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Vitronectin concentration was determined to be 234.2 µg/mL in serum.

    • Sandwich ELISA - Human Vitronectin/S-Protein ELISA Kit (ab234577)
      Sandwich ELISA - Human Vitronectin/S-Protein ELISA Kit (ab234577)
      To learn more about the advantages of recombinant antibodies see here.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab234577? Please let us know so that we can cite the reference in this datasheet.

    ab234577 has not yet been referenced specifically in any publications.

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