• Product name
    Human Von Willebrand Factor ELISA Kit (VWF)
    See all Von Willebrand Factor kits
  • Detection method
  • Precision
    Sample n Mean SD CV%
    Overall 5%
    Sample n Mean SD CV%
    Overall 7.1%
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    = 2.5 mU/ml
  • Range
    2.5 mU/ml - 80 mU/ml
  • Recovery

    99 %

  • Assay time
    5h 00m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Human Von Willebrand Factor (VWF) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Human Von Willebrand Factor in plasma, serum, and cell culture supernatants.

    A Von Willebrand Factor specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Von Willebrand Factor specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Von Willebrand Factor captured in plate.

    Get higher sensitivity in only 90 minutes with Human Von Willebrand Factor ELISA Kit (ab189571) from our SimpleStep ELISA® range.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform


  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent N Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 2 x 30ml
    50X Biotinylated Human Von Williebrand Factor Antibody 1 x 120µl
    Chromogen Substrate 1 x 8ml
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml
    Von Williebrand Factor Microplate (12 x 8 well strips) 1 unit
    Von Williebrand Factor Standard 1 x 2 vials
  • Research areas
  • Function
    Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.
  • Tissue specificity
  • Involvement in disease
    Defects in VWF are the cause of von Willebrand disease (VWD) [MIM:277480]. VWD defines a group of hemorrhagic disorders in which the von Willebrand factor is either quantitatively or qualitatively abnormal resulting in altered platelet function. Symptoms vary depending on severity and disease type but may include prolonged bleeding time, deficiency of factor VIII and impaired platelet adhesion. Type I von Willebrand disease is the most common form and is characterized by partial quantitative plasmatic deficiency of an otherwise structurally and functionally normal Willebrand factor; type II is associated with a qualitative deficiency and functional anomalies of the Willebrand factor; type III is the most severe form and is characterized by total or near-total absence of Willebrand factor in the plasma and cellular compartments, also leading to a profound deficiency of plasmatic factor VIII.
  • Sequence similarities
    Contains 1 CTCK (C-terminal cystine knot-like) domain.
    Contains 4 TIL (trypsin inhibitory-like) domains.
    Contains 3 VWFA domains.
    Contains 3 VWFC domains.
    Contains 4 VWFD domains.
  • Domain
    The von Willebrand antigen 2 is required for multimerization of vWF and for its targeting to storage granules.
  • Post-translational
    All cysteine residues are involved in intrachain or interchain disulfide bonds.
    N- and O-glycosylated.
  • Cellular localization
    Secreted. Secreted > extracellular space > extracellular matrix. Localized to storage granules.
  • Information by UniProt
  • Alternative names
    • Coagulation factor VIII
    • Coagulation factor VIII VWF
    • F8VWF
    • Factor VIII related antigen
    • von Willebrand antigen 2
    • von Willebrand antigen II
    • Von Willebrand disease
    • VWD
    • vWF
    see all
  • Database links


Our Abpromise guarantee covers the use of ab108918 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution.


  • VWF measured in biological fluids with background signal subtracted (duplicates +/- SD). There is limited cross-reactivity in mouse (< 2%) and rat samples (< 15%).

  • Representative Standard Curve using ab108918



This product has been referenced in:
  • Wang W  et al. Controlled release hydrogen sulfide delivery system based on mesoporous silica nanoparticles protects graft endothelium from ischemia-reperfusion injury. Int J Nanomedicine 11:3255-63 (2016). Read more (PubMed: 27486324) »
  • Liu Y  et al. The role of von Willebrand factor as a biomarker of tumor development in hepatitis B virus-associated human hepatocellular carcinoma: a quantitative proteomic based study. J Proteomics 106:99-112 (2014). Read more (PubMed: 24769235) »

See all 7 Publications for this product

Customer reviews and Q&As

1 IU/ml = 1000 mU/ml

I can confirm that the ELISA kits ab108918 and ab168548 are identical apart from ab168548 has a positive and negative plasma control included. The antibodies used in both of these kits are indeed the same.

Unfortunately I will not be able to send you a sample kit for testing purpose because this is against the company guidelines. All our product are vigorously tested in compatible samples and are fully guaranteed so if journal has any problem we can prov...

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Thank you for contacting us and making us aware of the typo in our protocol.

The corrected text on pg.8 of protocol last sentence of 3. Standard Curve which used to read: Diluent serves as the zero standard (0 µg/ml), now reads: “Diluent serv...

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Merci beaucoup pour votre émail et votre retour. Nous sommes très heureux de savoir que le problème s'est donc expliqué et résolu.

J'espère que vous avez bien reçu le kit complémentaire ...

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Merci de votre patience.

Voici les réponses à vos questions:

Le laboratoire à confirmé que c'est normal de voir des cristaux aussi dans le tampon du diluant. S'il y à des cristaux dans le tampon, ...

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Merci beaucoup pour votre émail.

Nous allons vous envoyer le kit lundi, vous devriez le recevoir donc mardi de la semaine prochaine. Veuillez s'il vous plaît nous tenir aussi au courant, sile problème est complèteme...

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Merci pour votre émail et votre patience.

Je peux vous confirmer qu'il s'agit d'une erreur d'annotation. Effectivement, les standards que vous avez reçu dans le kit lot GR79395 ne sont pas à 50mU, mais à 160mU. Read More

Merci beaucoup pour votre émail.
Je suis très désolée de ce problème que vous avez rencontré avec le standard du nouveau lot. Je peux vous assurer que nous prenons ceci très aux sérieux. Je suis en train d'investiguer ce problème et j'ai contacté ...

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Thank you for your inquiry.

The kit use purified vWF from plasma as standard. The standard has some factor VIII, but not a significant amount.

Please note that when you receive this kit, the concentration of the standard will be o...

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1-10 of 21 Abreviews or Q&A


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