Overview

  • Product name

    Human VTA1 ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Plasma 3.7%
    Inter-assay
    Sample n Mean SD CV%
    Plasma 8.3%
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    0.25 ng/ml
  • Range

    0.391 ng/ml - 25 ng/ml
  • Recovery

    98 %

  • Assay time

    4h 00m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat, Rabbit, Cow, Dog, Pig, Monkey
  • Product overview

    The Human VTA1 ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab222878) is designed for detection of human VTA1 in plasma, serum, and cell culture samples.


    This assay employs a quantitative sandwich enzyme immunoassay technique that measures human VTA1 in less than 4 hours. A polyclonal antibody specific for human VTA1 has been pre-coated onto a 96-well microplate with removable strips. VTA1 in standards and samples is sandwiched by the immobilized antibody and the biotinylated polyclonal antibody specific for VTA1, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • Notes

    Vacuolar protein sorting-associated protein VTA1 homolog (VTA1), also known as dopamine-responsive gene 1 protein (DRG-1), LYST-interacting protein 5 (LIP5), and SKD1-binding protein 1 (SBP1), is predominantly a cytosolic protein. VTA1 consists of 307 amino acids with a predicted molecular mass of 34 kDa. It is involved in the endosomal multivesicular body (MVB) formation and trafficking. The MVB is an endosomal compartment that serves to sort membrane proteins destined for degradation or routing to the lysosome. These membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids, are internalized into intraluminal vesicles that are generated by the invagination and scission from the limiting membrane of the endosome. The contents of the MVB are then transferred to lysosomes. VTA1 interacts with charged multivesicular body protein 5 (CHMP5) to form stable complexes in the cytoplasm, binds well to CHMP1B, CHMP2A, and CHMP3 proteins for MVB sorting, and is required for immunodeficiency virus type 1 HIV particle release.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Please refer to protocols.
  • Components 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent M Concentrate 1 x 20ml
    1X Standard Diluent 1 x 2ml
    20X Wash Buffer Concentrate 2 x 30ml
    Anti-Human VTA1 coated Microplate (12 x 8 wells) 1 unit
    Biotinylated Human VTA1 1 x 120µl
    Chromogen Substrate 1 x 8ml
    Human VTA1 Standard 1 x 2 vials
    Sealing Tapes 1 x 3 units
    Stop Solution 1 x 12ml
  • Research areas

  • Function

    Involved in the endosomal multivesicular bodies (MVB) pathway. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. Thought to be a cofactor of VPS4A/B, which catalyzes disassembles membrane-associated ESCRT-III assemblies. Involved in the sorting and down-regulation of EGFR (By similarity). Involved in HIV-1 budding.
  • Sequence similarities

    Belongs to the VTA1 family.
  • Cellular localization

    Cytoplasm. Endosome membrane.
  • Information by UniProt
  • Alternative names

    • C6orf55
    • Chromosome 6 open reading frame 55
    • Dopamine responsive protein DRG 1
    • Dopamine-responsive gene 1 protein
    • DRG 1
    • DRG-1
    • DRG1
    • Homolog of mouse SKD1 binding protein 1
    • HSPC228
    • LIP5
    • LYST interacting protein 5
    • LYST-interacting protein 5
    • My012
    • SBP1
    • SKD1 binding protein 1
    • SKD1-binding protein 1
    • Vacuolar protein sorting-associated protein VTA1 homolog
    • Vps20 associated 1 homolog (S. cerevisiae)
    • VTA1
    • VTA1_HUMAN
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab222878 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Example of human VTA1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Protocols

References

ab222878 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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