Overview

  • Product name

    Human Whole Blood Monocyte Isolation Kit
  • Sample type

    Whole Blood
  • Product overview

    Human Whole Blood Monocyte Isolation Kit (ab235700) allows for in vitro isolation of intact, viable monocytes. The kit enables the isolation of ≥ 5 x106 monocytes/mL yielding approximately 80% of the total monocytes present in 1 mL of whole blood


    The viability stain (included) used to identify living monocytes indicates that more than 99% of the cells in the isolated fraction are viable. The isolated fraction is pure - it contains more than 95% monocytes with less than 3% red blood cell counts.

  • Notes

    Monocytes are a type of white blood cell (leukocyte) and the largest of the immune cells. They are produced in the bone marrow and then migrate to the blood stream where they comprise 3-8% of all leukocytes. Depending on the clincial presentation of disease, monocytes will migrate to a specific tissue and then differentiate into a tissue-specific dendritic cell or a macrophage. Monocytes are easily identified by the large amoeboid shape, unilobar nucleus and vacuolated cytoplasm. They serve three important roles: phagocytosis, presentation of antigen to T cells which subsequently results in T cell clonal expansion, and the production of cytokines.

     

Properties

  • Storage instructions

    Please refer to protocols.
  • Components 30 ml
    54% Density Media 1 x 15ml
    Blunt-end needle 18 G; 1.5 in. (Sterile) 10 units
    Giemsa Stain 1 x 7ml
    Monocyte Density Gradient Media (MDGM) 1 x 25ml
    Monocyte Isolation Buffer (MIB) 1 x 115ml
    Viability Stain 1 x 200µl

Associated products

Images

  • The large, “fluffy” cytoplasm is approximately two-fold larger in size than the amoeboid nucleus (M, monocyte).

  • Total Cell Count (Left), Image from Fluorescent microscope with FITC/TRITC filters of same ROI showing live (green) and dead (red) cells (middle); merge of two panels (right).

Protocols

References

ab235700 has not yet been referenced specifically in any publications.

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