Product nameHuman Whole Blood Monocyte Isolation Kit
Sample typeWhole Blood
Human Whole Blood Monocyte Isolation Kit (ab235700) allows for in vitro isolation of intact, viable monocytes. The kit enables the isolation of ≥ 5 x106 monocytes/mL yielding approximately 80% of the total monocytes present in 1 mL of whole blood
The viability stain (included) used to identify living monocytes indicates that more than 99% of the cells in the isolated fraction are viable. The isolated fraction is pure - it contains more than 95% monocytes with less than 3% red blood cell counts.
Monocytes are a type of white blood cell (leukocyte) and the largest of the immune cells. They are produced in the bone marrow and then migrate to the blood stream where they comprise 3-8% of all leukocytes. Depending on the clincial presentation of disease, monocytes will migrate to a specific tissue and then differentiate into a tissue-specific dendritic cell or a macrophage. Monocytes are easily identified by the large amoeboid shape, unilobar nucleus and vacuolated cytoplasm. They serve three important roles: phagocytosis, presentation of antigen to T cells which subsequently results in T cell clonal expansion, and the production of cytokines.
Storage instructionsStore at +4°C. Please refer to protocols.
Components 30 ml 54% Density Media 1 x 15ml Blunt-end needle 18 G; 1.5 in. (Sterile) 10 units Giemsa Stain 1 x 7ml Monocyte Density Gradient Media (MDGM) 1 x 25ml Monocyte Isolation Buffer (MIB) 1 x 115ml Viability Stain 1 x 200µl
ab235700 has not yet been referenced specifically in any publications.