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|Sample type||Average %||Range|
|Cell culture supernatant||93.12||87% - 101%|
|Serum||90.42||80% - 97%|
|Plasma||127.1||119% - 135%|
Abcam's WISP-1 (CCN4) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of Human WISP-1 in serum, plasma (collect plasma using EDTA as an anticoagulant. Heparin and Citrate are not recommended as anticoagulants for use in this assay) and cell culture supernatants.
This assay employs an antibody specific for Human WISP-1coated on a 96-well plate. Standards and samples are pipetted into the wells and WISP-1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human WISP-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of WISP-1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
|Components||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 25ml|
|300X HRP-Streptavidin Concentrate||1 x 200µl|
|5X Assay Diluent B||1 x 15ml|
|Assay Diluent C||1 x 30ml|
|Biotinylated anti-Human WISP-1||2 vials|
|Recombinant Human WISP-1 Standard (lyophilized)||2 vials|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
|WISP-1 Microplate (12 x 8 wells)||1 unit|
Our Abpromise guarantee covers the use of ab155445 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab155445 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"