Human WISP-1 ELISA Kit (CCN4) (ab155445)

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Overview

  • Product name

    Human WISP-1 ELISA Kit (CCN4)

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 12%

  • Sample type

    Cell culture supernatant, Serum, Plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    < 50 pg/ml

  • Range

    0.061 ng/ml - 15 ng/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 93.12 87% - 101%
    Serum 90.42 80% - 97%
    Plasma 127.1 119% - 135%

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Abcam's WISP-1 (CCN4) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of Human WISP-1 in serum, plasma (collect plasma using EDTA as an anticoagulant. Heparin and Citrate are not recommended as anticoagulants for use in this assay) and cell culture supernatants.

    This assay employs an antibody specific for Human WISP-1coated on a 96-well plate. Standards and samples are pipetted into the wells and WISP-1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human WISP-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of WISP-1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applications

    Suitable for: Sandwich ELISAmore details

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    300X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B 1 x 15ml
    Assay Diluent C 1 x 30ml
    Biotinylated anti-Human WISP-1 2 vials
    Recombinant Human WISP-1 Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
    WISP-1 Microplate (12 x 8 wells) 1 unit

  • Research areas

  • Relevance

    The WISP-1/CCN4 gene encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: von Willebrand factor type C module, thrombospondin domain, insulin-like growth factor-binding domain and C-terminal cystine knot-like domain. This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. It is expressed at a high level in fibroblast cells, and overexpressed in colon tumors. The encoded protein binds to decorin and biglycan, two members of a family of small leucine-rich proteoglycans present in the extracellular matrix of connective tissue, and possibly prevents the inhibitory activity of decorin and biglycan in tumor cell proliferation. It also attenuates p53-mediated apoptosis in response to DNA damage through activation of the Akt kinase. It is 83% identical to the mouse protein at the amino acid level. Multiple alternatively spliced transcript variants have been identified.

  • Cellular localization

    Secreted

  • Alternative names

    • CCN family member 4
    • CCN4
    • WISP1
    • WISP1c
    • WISP1i
    • WISP1tc
    • Wnt 1 inducible signaling pathway protein 1
    • Wnt-1 induced secreted protein
    • WNT1 induced secreted protein 1
    • WNT1 inducible signaling pathway protein 1
    • WNT1 inducible-signaling pathway protein 1
    see all

  • Database links

    Applications

    Our Abpromise guarantee covers the use of ab155445 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Sandwich ELISA Use at an assay dependent concentration.

    Images

    • Typical Standard Curve
      Typical Standard Curve

      Representative standard curve using ab155445 -  Assay Diluent B.

    • Typical Standard Curve
      Typical Standard Curve

      Representative standard curve using ab155445 - Assay Diluent C.

    Protocols

    References

    This product has been referenced in:

    • Quiros M  et al. Macrophage-derived IL-10 mediates mucosal repair by epithelial WISP-1 signaling. J Clin Invest 127:3510-3520 (2017). Read more (PubMed: 28783045) »
    See 1 Publication for this product

    Publishing research using ab155445? Please let us know so that we can cite the reference in this datasheet.

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