Human WISP-1 ELISA Kit (CCN4) (ab155445)
Key features and details
- Sensitivity: 50 pg/ml
- Range: 0.061 ng/ml - 15 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
-
Product name
Human WISP-1 ELISA Kit (CCN4) -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 12% -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 50 pg/ml -
Range
0.061 ng/ml - 15 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 93.12 87% - 101% Serum 90.42 80% - 97% Plasma 127.1 119% - 135% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam's WISP-1 (CCN4) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of Human WISP-1 in serum, plasma (collect plasma using EDTA as an anticoagulant. Heparin and Citrate are not recommended as anticoagulants for use in this assay) and cell culture supernatants.
This assay employs an antibody specific for Human WISP-1coated on a 96-well plate. Standards and samples are pipetted into the wells and WISP-1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human WISP-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of WISP-1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
-
Notes
Optimization may be required with urine samples.
-
Platform
Microplate
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent C 1 x 30ml Biotinylated anti-Human WISP-1 2 vials Recombinant Human WISP-1 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml WISP-1 Microplate (12 x 8 wells) 1 unit -
Research areas
-
Relevance
WISP1 is a downstream regulator in the Wnt/Frizzled-signaling pathway and is associated with cell survival. WISP1 attentuates p53-mediated apoptosis in response to DNA damage through activation of AKT kinase and up-regulates the anti-apoptotic Bcl-X(L) protein. WISP1 is expressed in heart, kidney, lung, pancreas, placenta, ovary, small intestine and spleen. Overexpression is associated with several cancers including breast cancer and colon tumors. -
Cellular localization
Secreted -
Alternative names
- CCN 4
- CCN family member 4
- CCN4
see all -
Database links
- Entrez Gene: 8840 Human
- Omim: 603398 Human
- SwissProt: O95388 Human
- Unigene: 492974 Human
Associated products
Images
-
Typical Standard Curve
Representative standard curve using ab155445 - Assay Diluent B.
-
Typical Standard CurveRepresentative standard curve using ab155445 - Assay Diluent C.
References (1)
ab155445 has been referenced in 1 publication.
- Quiros M et al. Macrophage-derived IL-10 mediates mucosal repair by epithelial WISP-1 signaling. J Clin Invest 127:3510-3520 (2017). PubMed: 28783045
