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    human-wisp-1-elisa-kit-ccn4-ab155445.pdf

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Epigenetics and Nuclear Signaling DNA / RNA DNA Damage & Repair DNA Damage Response Other
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Human WISP-1 ELISA Kit (CCN4) (ab155445)

  • Datasheet
  • SDS
  • Protocol Booklet
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Typical Standard Curve
  • Typical Standard Curve

Key features and details

  • Sensitivity: 50 pg/ml
  • Range: 0.061 ng/ml - 15 ng/ml
  • Sample type: Cell culture supernatant, Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

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Protein
Recombinant human WISP1 protein (ab50041)

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Overview

  • Product name

    Human WISP-1 ELISA Kit (CCN4)
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 12%
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 50 pg/ml
  • Range

    0.061 ng/ml - 15 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 93.12 87% - 101%
    Serum 90.42 80% - 97%
    Plasma 127.1 119% - 135%
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam's WISP-1 (CCN4) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of Human WISP-1 in serum, plasma (collect plasma using EDTA as an anticoagulant. Heparin and Citrate are not recommended as anticoagulants for use in this assay) and cell culture supernatants.

    This assay employs an antibody specific for Human WISP-1coated on a 96-well plate. Standards and samples are pipetted into the wells and WISP-1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human WISP-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of WISP-1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    300X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B 1 x 15ml
    Assay Diluent C 1 x 30ml
    Biotinylated anti-Human WISP-1 2 vials
    Recombinant Human WISP-1 Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
    WISP-1 Microplate (12 x 8 wells) 1 unit
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • DNA Damage Response
    • Other
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Developmental Families
    • Other
    • Stem Cells
    • Signaling Pathways
    • Wnt
    • Secreted
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • DNA Damage ELISA kits
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Nuclear Signaling ELISA kits
  • Relevance

    WISP1 is a downstream regulator in the Wnt/Frizzled-signaling pathway and is associated with cell survival. WISP1 attentuates p53-mediated apoptosis in response to DNA damage through activation of AKT kinase and up-regulates the anti-apoptotic Bcl-X(L) protein. WISP1 is expressed in heart, kidney, lung, pancreas, placenta, ovary, small intestine and spleen. Overexpression is associated with several cancers including breast cancer and colon tumors.
  • Cellular localization

    Secreted
  • Alternative names

    • CCN 4
    • CCN family member 4
    • CCN4
    • WISP 1
    • WISP 1c
    • WISP 1i
    • WISP 1tc
    • WISP1c
    • WISP1i
    • WISP1tc
    • Wnt 1 induced secreted protein
    • WNT 1 induced secreted protein 1
    • WNT 1 inducible signaling pathway protein 1
    • Wnt 1 signaling pathway protein 1
    • Wnt-1 inducible signaling pathway protein 1
    • Wnt1 induced secreted protein
    • WNT1 induced secreted protein 1
    • WNT1 inducible signaling pathway protein 1
    • Wnt1 signaling pathway protein 1
    • WNT1-inducible-signaling pathway protein 1
    see all
  • Database links

    • Entrez Gene: 8840 Human
    • Omim: 603398 Human
    • SwissProt: O95388 Human
    • Unigene: 492974 Human

    Associated products

      Images

      • Typical Standard Curve
        Typical Standard Curve

        Representative standard curve using ab155445 -  Assay Diluent B.

      • Typical Standard Curve
        Typical Standard Curve

        Representative standard curve using ab155445 - Assay Diluent C.

      Protocols

      • Protocol Booklet

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (1)

      Publishing research using ab155445? Please let us know so that we can cite the reference in this datasheet.

      ab155445 has been referenced in 1 publication.

      • Quiros M  et al. Macrophage-derived IL-10 mediates mucosal repair by epithelial WISP-1 signaling. J Clin Invest 127:3510-3520 (2017). PubMed: 28783045

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