The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Blocking - Blocking peptide for Anti-Wnt3a antibody (ab28472)
- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions. - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer. - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent. - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised. - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Information available upon request.
Protein Wnt 3a Precursor
Wingless type MMTV integration site family member 3A
Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube.
Moderately expressed in placenta and at low levels in adult lung, spleen, and prostate.
Belongs to the Wnt family.
Secreted > extracellular space > extracellular matrix.
Song N et al. Sonic hedgehog-c-Jun N-terminal kinase-zinc finger protein Gli1 signaling protects against high glucose concentration-induced reactive oxygen species generation in human fibroblasts. Exp Ther Med15:5084-5090 (2018).
Read more (PubMed: 29805534) »
Wang Y et al. Activation of Wnt signaling reduces high-glucose mediated damages on skin fibroblast cells. Iran J Basic Med Sci20:944-950 (2017).
Read more (PubMed: 29085587) »