Associated products


Our Abpromise guarantee covers the use of ab23327 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Blocking - Blocking peptide for Anti-Wnt3a antibody (ab28472)

  • Form

  • Additional notes

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Information available upon request.

  • Reconstitution
    Reconstitute with 100 ul of deionised water (or equivalent).

General Info

  • Alternative names

    • Protein Wnt 3a Precursor
    • Protein Wnt-3a
    • Wingless type MMTV integration site family member 3A
    • Wnt 3a
    • wnt3a
    • WNT3A protein
    see all
  • Function

    Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube.
  • Tissue specificity

    Moderately expressed in placenta and at low levels in adult lung, spleen, and prostate.
  • Sequence similarities

    Belongs to the Wnt family.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt


This product has been referenced in:

  • Song N  et al. Sonic hedgehog-c-Jun N-terminal kinase-zinc finger protein Gli1 signaling protects against high glucose concentration-induced reactive oxygen species generation in human fibroblasts. Exp Ther Med 15:5084-5090 (2018). Read more (PubMed: 29805534) »
  • Wang Y  et al. Activation of Wnt signaling reduces high-glucose mediated damages on skin fibroblast cells. Iran J Basic Med Sci 20:944-950 (2017). Read more (PubMed: 29085587) »
See all 2 Publications for this product

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