Overview

  • Product name

    Human XCL1 ELISA Kit
    See all XCL1 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Supernatant 8 5.4%
    Inter-assay
    Sample n Mean SD CV%
    Supernatant 3 3.1%
  • Sample type

    Cell culture supernatant, Serum, Cell culture media, Hep Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    28.3 pg/ml
  • Range

    97.48 pg/ml - 2800 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 105 101% - 109%
    Serum 98 96% - 100%
    Cell culture media 90 89% - 92%
    Hep Plasma 87 85% - 88%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    XCL1 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of XCL1 protein in human serum, plasma (heparin), and cell culture supernatants.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB Development Solution is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    XCL1 (Lymphotactin) is a secreted glycosylated protein of C chemokine family. It plays a role in chemotactic activity of lymphocytes, but not monocytes or neutrophils. In the thymus, XCL1 mediates medullary accumulation of dendritic cells and contributes to T cell development which plays a role in establishment of self-tolerance. XCL2 is functionally related XCL1 homolog, these proteins share 98% amino acids sequence identity. 

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human XCL1 Capture Antibody 1 x 600µl
    10X Human XCL1 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml
    Human XCL1 Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent 50BS 1 x 20ml
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Relevance

    Human lymphotactin (Lptn)/XCL1, also named human SCM1 and ATAC, is a member of gamma or C subfamily of chemokines that lack two of the four invariant cysteine residues normally found in the CC and CXC chemokines and have an extended C-terminus. Human lymphotactin encodes a 114 amino acid precursor protein with a 21 amino acid predicted signal peptide. The gene for human and mouse lymphotactin has been mapped to chromosome 1. Human and mouse lymphotactin share approximately 60% amino acid sequence identity. The expression of lymphotactin is restricted to activated T cells such as activated CD8+ T cells and other class I MHC restricted T cells. Since lymphotactin is produced by lymphocytes and acts on lymphocytes, it is speculated that it is a messenger in T cell chemoattraction. Human lymphotactin is chemotactic for lymphocytes and NK cells. Its expression is abundant in some activated T cells such as activated CD8+ T cells and other class I MCH restricted T cells and is absent in CD4+ T cells. It has been shown that lymphotactin is a key regulator of lymphocyte motility and adhesion during acute allograft rejection. The specific receptor for lymphotactin is the orphan receptor XCR1 (GPR5). It has chemotactic activity for lymphocytes and natural killer cells but not for monocytes or neutrophils.
  • Cellular localization

    Secreted
  • Alternative names

    • ATAC
    • Chemokine (C motif) ligand 1
    • Cytokine SCM1
    • LPTN
    • LTN
    • Lymphotactin
    • Lymphotaxin
    • SCM1
    • SCM1 alpha
    • SCM1a
    • SCYC1
    • Small inducible cytokine C1
    • XC chemokine ligand 1
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab264620 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

  • Standard Curve comparison between human XCL1 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

  • The XCL1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • Peripheral blood mononuclear cells (PBMC) were cultured in the presence (stimulated) or absence of 50 ng/mL PMA and 1 µg/mL Ionomycin for 24 hours. Cell culture supernatants were collected and assayed for the presence of XCL1. The concentrations of XCL1 were measured in duplicates, interpolated from the XCL1 standard curves and corrected for sample dilution. Undiluted samples are as follows: PBMC cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean XCL1 concentration was determined to be 4,506 pg/mL in PMA + Ionomycin treated PBMC cell culture supernatant and not detectable in untreated samples (data not shown).

Protocols

References

ab264620 has not yet been referenced specifically in any publications.

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