Human XIAP ELISA Kit (API3) (ab155446)
Key features and details
- Sensitivity: 0.25 ng/ml
- Range: 2.5 ng/ml - 600 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse, Human
Overview
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Product name
Human XIAP ELISA Kit (API3) -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 12% -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 0.25 ng/ml -
Range
2.5 ng/ml - 600 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 95.15 87% - 103% Serum 82 74% - 90% Plasma 74.45 67% - 82% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse, Human -
Product overview
Abcam's XIAP Human ELISA kit (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of Human XIAP in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human XIAP coated on a 96-well plate. Standards and samples are pipetted into the wells and XIAP present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human XIAP antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of XIAP bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Notes
Optimization may be required with urine samples.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml 500X HRP-Streptavidin Concentrate 1 x 200µl Biotinylated anti-Human XIAP 2 vials Recombinant Human XIAP Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml XIAP Microplate (12 x 8 wells) 1 unit -
Research areas
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Relevance
The XIAP gene encodes a protein that belongs to a family of apoptotic suppressor proteins. Members of this family share a conserved motif termed, baculovirus IAP repeat and this is necessary for their anti-apoptotic function. This protein functions through binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2 and inhibits apoptosis induced by menadione, a potent inducer of free radicals, and interleukin 1-beta converting enzyme. This protein also inhibits at least two members of the caspase family of cell-death proteases, caspase-3 and caspase-7. Mutations in this gene are the cause of X-linked lymphoproliferative syndrome. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 2 and 11. -
Cellular localization
Cytoplasmic and Nuclear -
Alternative names
- API3
- baculoviral IAP repeat-containing 4
- Baculoviral IAP repeat-containing protein 4
see all -
Database links
- Entrez Gene: 331 Human
- Entrez Gene: 11798 Mouse
- Omim: 300079 Human
- SwissProt: P98170 Human
- SwissProt: Q60989 Mouse
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab155446 has not yet been referenced specifically in any publications.