Key features and details
- Unconjugated capture and detector antibodies
- Adaptable to any antibody pair-based assay format
- Antibody concentration ~ 1 mg/ml
- BSA and azide free buffer - ready for conjugation
- Reacts with: Mouse, Human
Product nameHuman/Mouse Myelin oligodendrocyte glycoprotein (MOG) Antibody Pair - BSA and Azide free
See all Myelin oligodendrocyte glycoprotein kits
Assay typeELISA set
Range6.25 pg/ml - 400 pg/ml
Species reactivityReacts with: Mouse, Human
Human/Mouse MOG Antibody Pair is a matched pair of unconjugated recombinant rabbit monoclonal capture and detection antibodies used to quantify Human/Mouse MOG in sandwich ELISAs and many other pair-based applications.
The pair can be used in variety of assays and platforms including but not limited to:
- Sandwich ELISA
- Meso Scale Discovery® (MSD®)
- Luminex® and bead-based assays
- DELFIA® immunoassays
- Simoa® and Single Molecule Counting (SMC™) immunoassays
Our antibody pairs are supplied in a carrier-free format that is conjugation-ready:
- Buffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.
- Concentration of ~1 mg/ml as measured by the protein A280 method.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for biotin, HRP, gold, and fluorescent dyes.
We can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation services.
Pairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.
The recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.
The range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.
We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.
Capture antibody: recombinant rabbit monoclonal (unconjugated) – 100 µg
Detector antibody: recombinant rabbit monoclonal (unconjugated) – 100 µg
Concentration: ~1 mg/ml
Storage buffer: 100% PBS
Recombinant monoclonal antibodies offer several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free production
For more information see here.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Meso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.
Luminex is a trademark of Luminex Corporation, registered in the US and other countries.
AlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.
Simoa is a registered trademark of Quanterix, Inc.
SMC is a registered trademark of Merck KGaA, Darmstadt, Germany.
Tested applicationsSuitable for: Sandwich ELISAmore details
Storage instructionsStore at +4°C. Please refer to protocols.
Components 10 x 96 tests Human/Mouse MOG Detector Antibody (unconjugated) 1 x 100µg Human/Mouse MOG Capture Antibody (unconjugated) 1 x 100µg
FunctionMediates homophilic cell-cell adhesion (By similarity). Minor component of the myelin sheath. May be involved in completion and/or maintenance of the myelin sheath and in cell-cell communication.
Tissue specificityFound exclusively in the CNS, where it is localized on the surface of myelin and oligodendrocyte cytoplasmic membranes.
Sequence similaritiesBelongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Cellular localizationCell membrane.
- Information by UniProt
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab253500 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use at an assay dependent concentration.
To learn more about the advantages of recombinant antibodies see here.
Representative standard curve from corresponding SimpleStep ELISA® Kit (ab278115), which uses the same antibody pair. For additional information on the performance of pair and kit, refer to the corresponding kit datasheet. Due to differences in the formulation and format of the antibodies in this pair, they cannot be used as substitutes for the antibody components in our SimpleStep ELISA® Kits.
Representative standard curve from corresponding SimpleStep ELISA® Kit (ab282304), which uses the same antibody pair. For additional information on the performance of pair and kit, refer to the corresponding kit datasheet. Due to differences in the formulation and format of the antibodies in this pair, they cannot be used as substitutes for the antibody components in our SimpleStep ELISA® Kits.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab253500 has not yet been referenced specifically in any publications.