Human PAR1/Thrombin Receptor ELISA Kit (ab283544)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.21 pg/ml
- Range: 3.125 pg/ml - 200 pg/ml
- Sample type: Cell culture extracts, Cell culture supernatant, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
-
Product name
Human PAR1/Thrombin Receptor ELISA Kit
See all PAR1/Thrombin Receptor kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% serum 8 2.6% Inter-assay Sample n Mean SD CV% serum 3 4.7% -
Sample type
Cell culture supernatant, Serum, Cell culture extracts, Hep Plasma, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1.21 pg/ml -
Range
3.125 pg/ml - 200 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 110 107% - 113% Serum 113 106% - 116% Hep Plasma 98 95% - 101% EDTA Plasma 109 107% - 112% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human PAR1/Thrombin Receptor ELISA kit (ab272199) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human PAR1/Thrombin Receptor protein in human serum, plasma, cell culture supernatant, and cell culture extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human PAR1/Thrombin Receptor with 1.21 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
-
Notes
Human PAR1/Thrombin Receptor also known as Protease-activated receptor 1 or coagulation factor II (thrombin) receptor is a protein that in humans is encoded by the F2R gene. Human PAR1 is a G protein-coupled receptor and one of four protease-activated receptors involved in the regulation of thrombotic response. Highly expressed in platelets and endothelial cells, human PAR1 plays a key role in mediating the interplay between coagulation and inflammation, which is important in the pathogenesis of inflammatory and fibrotic lung diseases. Human PAR1 is also involved both in disruption and maintenance of endothelial barrier integrity, through interaction with either thrombin or activated protein C, respectively.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human PAR1/Thrombin Receptor Capture Antibody 1 x 600µl 10X Human PAR1/Thrombin Receptor Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human PAR1/Thrombin Receptor Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
-
Function
High affinity receptor for activated thrombin coupled to G proteins that stimulate phosphoinositide hydrolysis. May play a role in platelets activation and in vascular development. -
Tissue specificity
Platelets and vascular endothelial cells. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Post-translational
modificationsA proteolytic cleavage generates a new N-terminus that functions as a tethered ligand.
Phosphorylated; probably mediating desensitization prior to the uncoupling and internalization of the receptor. -
Cellular localization
Cell membrane. - Information by UniProt
-
Alternative names
- CF2R
- Coagulation factor II (thrombin) receptor
- Coagulation factor II receptor
see all -
Database links
- Entrez Gene: 2149 Human
- Omim: 187930 Human
- SwissProt: P25116 Human
- Unigene: 482562 Human
Images
-
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
-
To learn more about the advantages of recombinant antibodies see here.
-
The PAR1/Thrombin Receptor standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
-
The concentrations of PAR1/Thrombin Receptor were measured in duplicates, interpolated from the PAR1/Thrombin Receptor standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (EDTA) 50%, plasma (heparin) 100%, HT-29 supernatant 50%, and A375 supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PAR1/Thrombin Receptor concentration was determined to be 420.97 pg/mL in serum, 464.59 pg/mL in plasma (EDTA), 97.80 pg/mL in plasma (heparin), 360.01 pg/mL in HT-29 supernatant and 28.52 pg/mL in A375 supernatant.
-
The concentrations of PAR1/Thrombin Receptor were measured in duplicate and interpolated from the PAR1/Thrombin Receptor standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PAR1/Thrombin Receptor concentration was determined to be 236.37 pg/mL in HT-29 cell extract.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab283544 has not yet been referenced specifically in any publications.