Key features and details
- Mouse monoclonal [F18-1G10.2] to Hunchback
- Suitable for: ICC/IF
- Reacts with: Drosophila melanogaster
- Isotype: IgG1
Product nameAnti-Hunchback antibody [F18-1G10.2]
DescriptionMouse monoclonal [F18-1G10.2] to Hunchback
Tested applicationsSuitable for: ICC/IFmore details
Species reactivityReacts with: Drosophila melanogaster
The details of the immunogen for this antibody are not available.
This antibody clone is manufactured by Abcam.
Product was previously marketed under the MitoSciences sub-brand.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: 99% HEPES buffered saline
Concentration information loading...
Light chain typekappa
Our Abpromise guarantee covers the use of ab197787 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
FunctionGap class segmentation protein that controls development of head structures.
Tissue specificityIn embryo, expression of maternal transcript is highest in anterior region. Zygotic transcript is expressed in anterior region until the beginning of gastrulation and in posterior region until early gastrulation. After this, it is expressed in developing nervous system.
Sequence similaritiesBelongs to the hunchback C2H2-type zinc-finger protein family.
Contains 6 C2H2-type zinc fingers.
Developmental stageExpressed maternally and zygotically. Expression of the maternal transcript decreases until embryonic stage 14, zygotic transcript is first detected at stage 11.
- Information by UniProt
- CG9786 antibody
- Dmel\CG9786 antibody
- hb antibody
Immunocytochemistry analysis using Hunchback mouse monoclonal F18-1G10 to detect Hunchback protein in all neuroblast nuclei from Drosophila stage 9 embryos. The cells were fixed in 4% formaldehyde for 25 minutes, permeabilized with 0.1% Triton X-100/PBS, and blocked in 0.1% Triton X-100/PBS, 2.5% Normal Goat Serum, 2.5% Normal Donkey Serum. The Hunchback stain used mouse monoclonal F18-1G10 at 10 µg/mL and was detected with Alexa555 conjugated goat anti-mouse (at 10 µg/mL).
ab197787 has not yet been referenced specifically in any publications.