Overview

  • Product name

  • Description

    Rabbit polyclonal to Huntingtin
  • Host species

    Rabbit
  • Specificity

    Human HTT caspase cleavage sites generate fragment-specific forms of the protein. Caspase-3/7 has been shown to generate cleavage sites at animo acids 513 and 552. Caspase-2 cleaves at amino acid 552 and caspase-6 at amino acid 586. Neo-specific antibody ab155930 recognizes the 586 cleaved fragment without detecting the full-length form.
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Fr, Sandwich ELISAmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide:

    C-PSDSSEIVLD

    conjugated to KLH by a Cysteine residue linker, corresponding to amino acids 575-584 of Human Huntingtin (P42858).

  • Positive control

    • Transfected 293T cells overexpressing Huntingtin constructs; Tissues (Striatum and cortex) from transgenic HD mice of the YAC128 line.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 99% PBS, 0.1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Neoepitope antibodies distinguish smaller cleaved fragments or processed forms of proteins versus the intact full-length or precursor by using a designed peptide purification process to maximize immunoreactivity to a specific cleavage site.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab155930 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Predicted molecular weight: 348 kDa.
ICC/IF 1/50 - 1/200.
IHC-Fr 1/50 - 1/200.
Sandwich ELISA 1/20 - 1/100.

Target

  • Function

    May play a role in microtubule-mediated transport or vesicle function.
  • Tissue specificity

    Expressed in the brain cortex (at protein level). Widely expressed with the highest level of expression in the brain (nerve fibers, varicosities, and nerve endings). In the brain, the regions where it can be mainly found are the cerebellar cortex, the neocortex, the striatum, and the hippocampal formation.
  • Involvement in disease

    Defects in HTT are the cause of Huntington disease (HD) [MIM:143100]. HD is an autosomal dominant neurodegenerative disorder characterized by involuntary movements (chorea), general motor impairment, psychiatric disorders and dementia. Onset of the disease occurs usually in the third or fourth decade of life and symptoms progressively worsen leading to death in 10 to 20 years. Onset and clinical course depend on the degree of poly-Gln repeat expansion, longer expansions resulting in earlier onset and more severe clinical manifestations. HD affects 1 in 10,000 individuals of European origin. Neuropathology of Huntington disease displays a distinctive pattern with loss of neurons, especially in the caudate and putamen (striatum).
  • Sequence similarities

    Belongs to the huntingtin family.
    Contains 10 HEAT repeats.
  • Domain

    The N-terminal Gln-rich and Pro-rich domain has great conformational flexibility and is likely to exist in a fluctuating equilibrium of alpha-helical, random coil, and extended conformations.
  • Post-translational
    modifications

    Cleaved by apopain downstream of the polyglutamine stretch. The resulting N-terminal fragment is cytotoxic and provokes apoptosis.
    Forms with expanded polyglutamine expansion are specifically ubiquitinated by SYVN1, which promotes their proteasomal degradation.
  • Cellular localization

    Cytoplasm. Nucleus. The mutant Huntingtin protein colocalizes with AKAP8L in the nuclear matrix of Huntington's disease neurons.
  • Information by UniProt
  • Database links

  • Alternative names

    • AI256365 antibody
    • C430023I11Rik antibody
    • HD antibody
    • HD protein antibody
    • HD_HUMAN antibody
    • HDH antibody
    • HTT antibody
    • Huntingtin antibody
    • HUNTINGTON CHOREA antibody
    • Huntington disease protein antibody
    • Huntington's disease protein homolog antibody
    • IT 15 antibody
    • IT15 antibody
    • OTTMUSP00000026909 antibody
    • ZHD antibody
    see all

Images

  • All lanes : Anti-Huntingtin antibody (ab155930) at 1/500 dilution

    Lane 1 : Lysate containing endogenous Huntingtin without capase activity
    Lane 2 : Lysate containing endogenous Huntingtin with capase2 activity
    Lane 3 : Lysate containing endogenous Huntingtin with capase3 activity
    Lane 4 : Lysate containing endogenous Huntingtin with capase6 activity
    Lane 5 : Lysate containing endogenous Huntingtin with capase7 activity
    Lane 6 : Lysate containing overexpressed recombinant Huntingtin fragment (aa1-513)
    Lane 7 : Lysate containing overexpressed recombinant Huntingtin fragment (aa1-536)
    Lane 8 : Lysate containing overexpressed recombinant Huntingtin fragment (aa1-552)
    Lane 9 : Lysate containing overexpressed recombinant Huntingtin fragment (aa1-586)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lanes 1-7 & 9 : Goat anti-rabbit HRP conjugated antibody at 1/30000 dilution
    Lane 8 : Goat anti-rabbit HRP conjugated antibody at 1/30000 dilution

    Developed using the ECL technique.

    Predicted band size: 348 kDa



    SDS-PAGE: 4-12% Bis-Tris polyacrylamide gel
  • Sandwich ELISA was performed with a monoclonal antibody to Huntingtin and ab155930 to determine the antigen concentration of the Huntingtin cleavage products. The curve represents a dose response for neo586 in 293T cells overexpressing the Huntingtin construct.
  • Immunofluorescent analysis of formalin fixed, permeabilized 293T cells transfected with Huntingtin23Q (left panel) and Huntingtin148Q (right panel)stop constructs ending in amino acid 586 labeling capase cleaved Huntingtin with ab155930 at 1/50(green). Nuclei were stained with Hoechst 33342 (blue).

References

ab155930 has not yet been referenced specifically in any publications.

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