Anti-Huntingtin Associated Protein 1 antibody [1B6] (ab39713)
Key features and details
- Mouse monoclonal [1B6] to Huntingtin Associated Protein 1
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG1
Overview
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Product name
Anti-Huntingtin Associated Protein 1 antibody [1B6] -
Description
Mouse monoclonal [1B6] to Huntingtin Associated Protein 1 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Monkey -
Immunogen
Fusion protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ab39713 gave a strong signal in Mouse Brain Tissue Lysate. The same band was detected in Rat Brain Tissue Lysate, although at a lower intensity.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
1B6 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab39713 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Notes |
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Immunohistochemistry (PFA fixed): Use at an assay dependent dilution.
IHC-FrFl: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: 1/100 - 1/1000. Detects a band of approximately 85 kDa (predicted molecular weight: 75 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Associates specifically with huntingtin. This binding is enhanced by an expanded polyglutamine repeat. -
Tissue specificity
Predominantly expressed in brain. Selectively expressed in neurons. -
Sequence similarities
Contains 1 HAP1 N-terminal domain. - Information by UniProt
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Database links
- Entrez Gene: 15114 Mouse
- Entrez Gene: 29430 Rat
- SwissProt: O35668 Mouse
- SwissProt: P54256 Rat
- Unigene: 281700 Mouse
- Unigene: 37430 Rat
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Alternative names
- HAP 1 antibody
- HAP 1A antibody
- HAP 2 antibody
see all
Images
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All lanes : Anti-Huntingtin Associated Protein 1 antibody [1B6] (ab39713) at 1 µg/ml
Lane 1 : Brain (Rat) Tissue Lysate - normal tissue
Lane 2 : Brain (Mouse) Tissue Lysate - normal tissue
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 75 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?
Additional bands at: 50 kDa (possible non-specific secondary antibody binding)
ab39713 detects a band at 85 kDa in mouse and rat brain tissue lysate. This band corresponds to the size of the HAP1-B protein detected in rat brain tissue lysate by Shi-Hua Li et al. (1998). (PMID: 9798945).
Datasheets and documents
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Datasheet download
References (3)
ab39713 has been referenced in 3 publications.
- Torre ER et al. A protocol for isolation and biochemical characterization of stigmoid bodies from rat brain. J Neurosci Methods 125:27-32 (2003). PubMed: 12763227
- Gutekunst CA et al. Stigmoid bodies contain type I receptor proteins SorLA/LR11 and sortilin: new perspectives on their function. J Histochem Cytochem 51:841-52 (2003). PubMed: 12754295
- Chan EY et al. Targeted disruption of Huntingtin-associated protein-1 (Hap1) results in postnatal death due to depressed feeding behavior. Hum Mol Genet 11:945-59 (2002). PubMed: 11971876