Product nameAnti-Huntingtin Associated Protein 1 antibody [1B6]
See all Huntingtin Associated Protein 1 primary antibodies
DescriptionMouse monoclonal [1B6] to Huntingtin Associated Protein 1
SpecificityRecognises both A and B isoforms of Huntingtin Associated Protein 1 (HAP1)
Tested applicationsSuitable for: IHC (PFA fixed), IHC-FrFl, ICC/IF, WB, IPmore details
Species reactivityReacts with: Mouse, Rat, Monkey
Fusion protein corresponding to Rat Huntingtin Associated Protein 1.
- ab39713 gave a strong signal in Mouse Brain Tissue Lysate. The same band was detected in Rat Brain Tissue Lysate, although at a lower intensity.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.4
Concentration information loading...
Light chain typekappa
Our Abpromise guarantee covers the use of ab39713 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC (PFA fixed)|
Immunohistochemistry (PFA fixed): Use at an assay dependent dilution.
IHC-FrFl: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: 1/100 - 1/1000. Detects a band of approximately 85 kDa (predicted molecular weight: 75 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionAssociates specifically with huntingtin. This binding is enhanced by an expanded polyglutamine repeat.
Tissue specificityPredominantly expressed in brain. Selectively expressed in neurons.
Sequence similaritiesContains 1 HAP1 N-terminal domain.
- Information by UniProt
- HAP 1 antibody
- HAP 1A antibody
- HAP 2 antibody
All lanes : Anti-Huntingtin Associated Protein 1 antibody [1B6] (ab39713) at 1 µg/ml
Lane 1 : Brain (Rat) Tissue Lysate - normal tissue
Lane 2 : Brain (Mouse) Tissue Lysate - normal tissue
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 75 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?
Additional bands at: 50 kDa (possible non-specific secondary antibody binding)
ab39713 detects a band at 85 kDa in mouse and rat brain tissue lysate. This band corresponds to the size of the HAP1-B protein detected in rat brain tissue lysate by Shi-Hua Li et al. (1998). (PMID: 9798945).
This product has been referenced in:
- Torre ER et al. A protocol for isolation and biochemical characterization of stigmoid bodies from rat brain. J Neurosci Methods 125:27-32 (2003). Read more (PubMed: 12763227) »
- Gutekunst CA et al. Stigmoid bodies contain type I receptor proteins SorLA/LR11 and sortilin: new perspectives on their function. J Histochem Cytochem 51:841-52 (2003). Read more (PubMed: 12754295) »