Recombinant Anti-HIP1 antibody [EPR10814] (ab181238)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10814] to HIP1
- Suitable for: ICC/IF, WB, IP
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
-
Product name
Anti-HIP1 antibody [EPR10814]
See all HIP1 primary antibodies -
Description
Rabbit monoclonal [EPR10814] to HIP1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab188140) -
Positive control
- WB: HAP1, HCT-116, HeLa, Jurkat and A549 cell lysates. ICC/IF: HeLa cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
-
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10814 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181238 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
1/100 - 1/250.
|
|
WB |
1/1000 - 1/10000. Detects a band of approximately 116 kDa (predicted molecular weight: 116 kDa).
|
|
IP |
1/20 - 1/40.
|
Notes |
---|
ICC/IF
1/100 - 1/250. |
WB
1/1000 - 1/10000. Detects a band of approximately 116 kDa (predicted molecular weight: 116 kDa). |
IP
1/20 - 1/40. |
Target
-
Function
Plays a role in clathrin-mediated endocytosis and trafficking. Involved in regulating AMPA receptor trafficking in the central nervous system in an NMDA-dependent manner. Enhances androgen receptor (AR)-mediated transcription. May act as a proapoptotic protein that induces cell death by acting through the intrinsic apoptosis pathway. Binds 3-phosphoinositides (via ENTH domain). May act through the ENTH domain to promote cell survival by stabilizing receptor tyrosine kinases following ligand-induced endocytosis. May play a functional role in the cell filament networks. May be required for differentiation, proliferation, and/or survival of somatic and germline progenitors. -
Tissue specificity
Ubiquitously expressed with the highest level in brain. Expression is up-regulated in prostate and colon cancer. -
Involvement in disease
Note=A chromosomal aberration involving HIP1 is found in a form of chronic myelomonocytic leukemia (CMML). Translocation t(5;7)(q33;q11.2) with PDGFRB. The chimeric HIP1-PDGFRB transcript results from an in-frame fusion of the two genes. The reciprocal PDGFRB-HIP1 transcript is not expressed. -
Sequence similarities
Belongs to the SLA2 family.
Contains 1 ENTH (epsin N-terminal homology) domain.
Contains 1 I/LWEQ domain. -
Domain
The pseudo DED region (pDED) mediates the interaction with IFT57.
Binds F-actin via the talin-like I/LWEQ domain. -
Cellular localization
Cytoplasm. Nucleus. Endomembrane system. Cytoplasmic vesicle > clathrin-coated vesicle membrane. Shuttles between cytoplasm and nucleus. Nuclear translocation can be induced by AR. - Information by UniProt
-
Database links
- Entrez Gene: 3092 Human
- Entrez Gene: 215114 Mouse
- Entrez Gene: 192154 Rat
- Omim: 601767 Human
- SwissProt: O00291 Human
- SwissProt: Q8VD75 Mouse
- Unigene: 329266 Human
- Unigene: 619089 Human
see all -
Alternative names
- 2610109B09Rik antibody
- A930014B11Rik antibody
- E130315I21Rik antibody
see all
Images
-
All lanes : Anti-HIP1 antibody [EPR10814] (ab181238) at 1/1000 dilution
Lane 1 : wild-type HAP1 cell lysate
Lane 2 : HIP1 knockout HAP1 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 116 kDa
Observed band size: 116 kDaLanes 1 - 3: Merged signal (red and green). Green - ab181238 observed at 116 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab181238 was shown to react with HIP1 in wild-type HAP1 cells in western blot with loss of signal observed in HIP1 knockout sample. Wild-type and HIP1 knockout HAP1 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab181238 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
-
Immunofluorescent analysis of HeLa cells (-20℃ Acetone-fixed) labeling HIP1 with ab181238 at 1/250 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).
-
All lanes : Anti-HIP1 antibody [EPR10814] (ab181238) at 1/2000 dilution
Lane 1 : HCT-116 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 116 kDa -
Western blot analysis of immunoprecipitation pellet from A549 cell lysate immunoprecipitated using ab181238 at 1/50 dilution.
Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (3)
ab181238 has been referenced in 3 publications.
- Azarnia Tehran D et al. Selective endocytosis of Ca2+-permeable AMPARs by the Alzheimer's disease risk factor CALM bidirectionally controls synaptic plasticity. Sci Adv 8:eabl5032 (2022). PubMed: 35613266
- Bettencourt C et al. MOBP and HIP1 in multiple system atrophy: New α-synuclein partners in glial cytoplasmic inclusions implicated in the disease pathogenesis. Neuropathol Appl Neurobiol 47:640-652 (2021). PubMed: 33368549
- Yang Q et al. Endocytic Adaptor Protein HIP1R Controls Intracellular Trafficking of Epidermal Growth Factor Receptor in Neuronal Dendritic Development. Front Mol Neurosci 11:447 (2018). PubMed: 30574069