Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Huntingtin Interacting Protein HIP1 aa 950 to the C-terminus. The exact sequence is proprietary. Database link: O00291
HCT-116, HeLa, Jurkat and A549 cell lysates; HeLa cells.
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This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/250.
1/1000 - 1/10000. Detects a band of approximately 116 kDa (predicted molecular weight: 116 kDa).
1/20 - 1/40.
Plays a role in clathrin-mediated endocytosis and trafficking. Involved in regulating AMPA receptor trafficking in the central nervous system in an NMDA-dependent manner. Enhances androgen receptor (AR)-mediated transcription. May act as a proapoptotic protein that induces cell death by acting through the intrinsic apoptosis pathway. Binds 3-phosphoinositides (via ENTH domain). May act through the ENTH domain to promote cell survival by stabilizing receptor tyrosine kinases following ligand-induced endocytosis. May play a functional role in the cell filament networks. May be required for differentiation, proliferation, and/or survival of somatic and germline progenitors.
Ubiquitously expressed with the highest level in brain. Expression is up-regulated in prostate and colon cancer.
Involvement in disease
Note=A chromosomal aberration involving HIP1 is found in a form of chronic myelomonocytic leukemia (CMML). Translocation t(5;7)(q33;q11.2) with PDGFRB. The chimeric HIP1-PDGFRB transcript results from an in-frame fusion of the two genes. The reciprocal PDGFRB-HIP1 transcript is not expressed.
Belongs to the SLA2 family. Contains 1 ENTH (epsin N-terminal homology) domain. Contains 1 I/LWEQ domain.
The pseudo DED region (pDED) mediates the interaction with IFT57. Binds F-actin via the talin-like I/LWEQ domain.
Cytoplasm. Nucleus. Endomembrane system. Cytoplasmic vesicle > clathrin-coated vesicle membrane. Shuttles between cytoplasm and nucleus. Nuclear translocation can be induced by AR.
Immunocytochemistry/ Immunofluorescence - Anti-Huntingtin Interacting Protein HIP1 antibody [EPR10814] (ab181238)
Immunofluorescent analysis of HeLa cells (-20℃ Acetone-fixed) labeling Huntingtin Interacting Protein HIP1 with ab181238 at 1/250 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).
Immunoprecipitation - Anti-Huntingtin Interacting Protein HIP1 antibody [EPR10814] (ab181238)
Western blot analysis of immunoprecipitation pellet from A549 cell lysate immunoprecipitated using ab181238 at 1/50 dilution. Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.
Western blot - Anti-Huntingtin Interacting Protein HIP1 antibody [EPR10814] (ab181238)
All lanes : Anti-Huntingtin Interacting Protein HIP1 antibody [EPR10814] (ab181238) at 1/2000 dilution
Lane 1 : HCT-116 cell lysate Lane 2 : HeLa cell lysate Lane 3 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Secondary All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution