Anti-Huntingtin (phospho S421) antibody (ab118827)
Key features and details
- Rabbit polyclonal to Huntingtin (phospho S421)
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Huntingtin (phospho S421) antibody
See all Huntingtin primary antibodies -
Description
Rabbit polyclonal to Huntingtin (phospho S421) -
Host species
Rabbit -
Specificity
ab118827 is specific to Huntingtin phosphorylated at Ser421. -
Tested Applications & Species
Application Species IHC-P Human -
Immunogen
Synthetic peptide:
RSGSIVELI
conjugated to KLH, corresponding to amino acids 416-424 of Human Huntingtin phosphorylated at Ser421 (NP_002102.4). -
Positive control
- Human brain, cerebellum tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.424% Potassium phosphate, 0.88% Sodium chloride -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab118827 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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IHC-P |
Human
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All applications |
Mouse
Rat
|
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use a concentration of 10 - 20 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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IHC-P
Use a concentration of 10 - 20 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
May play a role in microtubule-mediated transport or vesicle function. -
Tissue specificity
Expressed in the brain cortex (at protein level). Widely expressed with the highest level of expression in the brain (nerve fibers, varicosities, and nerve endings). In the brain, the regions where it can be mainly found are the cerebellar cortex, the neocortex, the striatum, and the hippocampal formation. -
Involvement in disease
Defects in HTT are the cause of Huntington disease (HD) [MIM:143100]. HD is an autosomal dominant neurodegenerative disorder characterized by involuntary movements (chorea), general motor impairment, psychiatric disorders and dementia. Onset of the disease occurs usually in the third or fourth decade of life and symptoms progressively worsen leading to death in 10 to 20 years. Onset and clinical course depend on the degree of poly-Gln repeat expansion, longer expansions resulting in earlier onset and more severe clinical manifestations. HD affects 1 in 10,000 individuals of European origin. Neuropathology of Huntington disease displays a distinctive pattern with loss of neurons, especially in the caudate and putamen (striatum). -
Sequence similarities
Belongs to the huntingtin family.
Contains 10 HEAT repeats. -
Domain
The N-terminal Gln-rich and Pro-rich domain has great conformational flexibility and is likely to exist in a fluctuating equilibrium of alpha-helical, random coil, and extended conformations. -
Post-translational
modificationsCleaved by apopain downstream of the polyglutamine stretch. The resulting N-terminal fragment is cytotoxic and provokes apoptosis.
Forms with expanded polyglutamine expansion are specifically ubiquitinated by SYVN1, which promotes their proteasomal degradation. -
Cellular localization
Cytoplasm. Nucleus. The mutant Huntingtin protein colocalizes with AKAP8L in the nuclear matrix of Huntington's disease neurons. - Information by UniProt
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Database links
- Entrez Gene: 3064 Human
- Entrez Gene: 15194 Mouse
- Entrez Gene: 29424 Rat
- Omim: 143100 Human
- Omim: 613004 Human
- SwissProt: P42858 Human
- SwissProt: P42859 Mouse
- SwissProt: P51111 Rat
see all -
Alternative names
- AI256365 antibody
- C430023I11Rik antibody
- HD antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Huntingtin (phospho S421) antibody (ab118827)ab118827, at 10-20 µg/ml, staining Huntingtin (phospho S421) in formalin fixed, paraffin embedded Human brain cerebellum tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab118827 has not yet been referenced specifically in any publications.