Overview

  • Product name
    Anti-Huntingtin (phospho S434) antibody
    See all Huntingtin primary antibodies
  • Description
    Rabbit polyclonal to Huntingtin (phospho S434)
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human Huntingtin aa 427-438 (phospho S432) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Sequence:

    GGSS CSPVLSRK

  • Positive control
    • Lysates of 293T cells transfected with Huntingtin and treated with PPI inhibitor and/or CDK5.

Properties

Applications

Our Abpromise guarantee covers the use of ab155931 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/200.
WB 1/250 - 1/500. Predicted molecular weight: 348 kDa.
ICC/IF 1/100 - 1/200.

Target

  • Function
    May play a role in microtubule-mediated transport or vesicle function.
  • Tissue specificity
    Expressed in the brain cortex (at protein level). Widely expressed with the highest level of expression in the brain (nerve fibers, varicosities, and nerve endings). In the brain, the regions where it can be mainly found are the cerebellar cortex, the neocortex, the striatum, and the hippocampal formation.
  • Involvement in disease
    Defects in HTT are the cause of Huntington disease (HD) [MIM:143100]. HD is an autosomal dominant neurodegenerative disorder characterized by involuntary movements (chorea), general motor impairment, psychiatric disorders and dementia. Onset of the disease occurs usually in the third or fourth decade of life and symptoms progressively worsen leading to death in 10 to 20 years. Onset and clinical course depend on the degree of poly-Gln repeat expansion, longer expansions resulting in earlier onset and more severe clinical manifestations. HD affects 1 in 10,000 individuals of European origin. Neuropathology of Huntington disease displays a distinctive pattern with loss of neurons, especially in the caudate and putamen (striatum).
  • Sequence similarities
    Belongs to the huntingtin family.
    Contains 10 HEAT repeats.
  • Domain
    The N-terminal Gln-rich and Pro-rich domain has great conformational flexibility and is likely to exist in a fluctuating equilibrium of alpha-helical, random coil, and extended conformations.
  • Post-translational
    modifications
    Cleaved by apopain downstream of the polyglutamine stretch. The resulting N-terminal fragment is cytotoxic and provokes apoptosis.
    Forms with expanded polyglutamine expansion are specifically ubiquitinated by SYVN1, which promotes their proteasomal degradation.
  • Cellular localization
    Cytoplasm. Nucleus. The mutant Huntingtin protein colocalizes with AKAP8L in the nuclear matrix of Huntington's disease neurons.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI256365 antibody
    • C430023I11Rik antibody
    • HD antibody
    • HD protein antibody
    • HD_HUMAN antibody
    • HDH antibody
    • HTT antibody
    • Huntingtin antibody
    • HUNTINGTON CHOREA antibody
    • Huntington disease protein antibody
    • Huntington's disease protein homolog antibody
    • IT 15 antibody
    • IT15 antibody
    • OTTMUSP00000026909 antibody
    • ZHD antibody
    see all

Images

  • All lanes : Anti-Huntingtin (phospho S434) antibody (ab155931) at 1/500 dilution

    Lane 1 : Lysate of 293T transfected with Huntingtin
    Lane 2 : Lysate of 293T transfected with Huntingtin and treated with PPI inhibitor
    Lane 3 : Lysate of 293T transfected with Huntingtin and treated with PPI inhibitor and CDK5

    Secondary
    All lanes : Goat anti-rabbit HRP conjugated antibody at 1/30000 dilution

    Developed using the ECL technique.

    Predicted band size: 348 kDa



    SDS PAGE: 4-20% Tris-HCl polyacrylamide gel

References

ab155931 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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