Anti-hUPF2/RENT2 antibody (ab157108)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-hUPF2/RENT2 antibody
See all hUPF2/RENT2 primary antibodies -
Description
Rabbit polyclonal to hUPF2/RENT2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Ferret, Gorilla, Opossum, Platypus
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Immunogen
Synthetic peptide, corresponding to a region within amino acids 1-50 of Human hUPF2/RENT2 (NP_056357.1).
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Positive control
- 293T, HeLa, Jurkat, NIH 3T3, TCMK and 4T1 whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab157108 was affinity purified using an epitope specific to hUPF2/RENT2 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab157108 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/5000. Predicted molecular weight: 148 kDa. | |
| IP | Use at 2-10 µg/mg of lysate. |
Target
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Function
Part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Binds spliced mRNA. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Contains 3 MIF4G domains. -
Cellular localization
Cytoplasm > perinuclear region. - Information by UniProt
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Database links
- Entrez Gene: 450304 Chimpanzee
- Entrez Gene: 781181 Cow
- Entrez Gene: 487130 Dog
- Entrez Gene: 100069342 Horse
- Entrez Gene: 26019 Human
- Entrez Gene: 326622 Mouse
- Omim: 605529 Human
- SwissProt: Q9HAU5 Human
see all -
Alternative names
- DKFZp434D222 antibody
- hUpf2 antibody
- KIAA1408 antibody
see all
Images
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Western blot - Anti-hUPF2/RENT2 antibody (ab157108)All lanes : Anti-hUPF2/RENT2 antibody (ab157108) at 0.4 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 148 kDa
Exposure time: 30 seconds -
Western blot - Anti-hUPF2/RENT2 antibody (ab157108)All lanes : Anti-hUPF2/RENT2 antibody (ab157108) at 1 µg/ml
Lane 1 : NIH 3T3 whole cell lysate
Lane 2 : TCMK whole cell lysate
Lane 3 : 4T1 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 148 kDa
Exposure time: 10 seconds -
Immunoprecipitation - Anti-hUPF2/RENT2 antibody (ab157108)Detection of hUPF2/RENT2 in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab157108 at 6 µg/mg lysate for IP (Lane 1). For WB detection an ab157108 was used at 1 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.
Datasheets and documents
References
ab157108 has not yet been referenced specifically in any publications.
