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    hupf2rent2-antibody-ab157108.pdf

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Epigenetics and Nuclear Signaling RNAi miRNA processing
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Anti-hUPF2/RENT2 antibody (ab157108)

  • Datasheet
  • SDS
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Western blot - Anti-hUPF2/RENT2 antibody (ab157108)
  • Western blot - Anti-hUPF2/RENT2 antibody (ab157108)
  • Immunoprecipitation - Anti-hUPF2/RENT2 antibody (ab157108)

Key features and details

  • Rabbit polyclonal to hUPF2/RENT2
  • Suitable for: WB, IP
  • Reacts with: Mouse, Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-hUPF2/RENT2 antibody
    See all hUPF2/RENT2 primary antibodies
  • Description

    Rabbit polyclonal to hUPF2/RENT2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Ferret, Gorilla, Opossum, Platypus
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 1-50 of Human hUPF2/RENT2 (NP_056357.1).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • 293T, HeLa, Jurkat, NIH 3T3, TCMK and 4T1 whole cell lysates.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab157108 was affinity purified using an epitope specific to hUPF2/RENT2 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • RNAi
    • miRNA processing

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • Jurkat whole cell lysate (ab30128)
    • NIH/3T3 whole cell lysate (ab7179)
    • Jurkat whole cell lysate (ab7899)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab157108 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/1000 - 1/5000. Predicted molecular weight: 148 kDa.
IP
Use at 2-10 µg/mg of lysate.
Notes
WB
1/1000 - 1/5000. Predicted molecular weight: 148 kDa.
IP
Use at 2-10 µg/mg of lysate.

Target

  • Function

    Part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Binds spliced mRNA.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Contains 3 MIF4G domains.
  • Cellular localization

    Cytoplasm > perinuclear region.
  • Target information above from: UniProt accession Q9HAU5 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 450304 Chimpanzee
    • Entrez Gene: 781181 Cow
    • Entrez Gene: 487130 Dog
    • Entrez Gene: 100069342 Horse
    • Entrez Gene: 26019 Human
    • Entrez Gene: 326622 Mouse
    • Omim: 605529 Human
    • SwissProt: Q9HAU5 Human
    • Unigene: 370689 Human
    • Unigene: 610110 Human
    • Unigene: 31102 Mouse
    see all
  • Alternative names

    • DKFZp434D222 antibody
    • hUpf2 antibody
    • KIAA1408 antibody
    • MGC138834 antibody
    • MGC138835 antibody
    • Nonsense mRNA reducing factor 2 antibody
    • Regulator of nonsense transcripts 2 antibody
    • RENT2 antibody
    • RENT2_HUMAN antibody
    • Smg 3 antibody
    • Smg 3 homolog nonsense mediated mRNA decay factor antibody
    • Up-frameshift suppressor 2 homolog antibody
    • UPF2 antibody
    • UPF2 regulator of nonsense transcripts homolog antibody
    • UROC1 antibody
    • Urocanate hydratase antibody
    • Yeast Upf2p homolog antibody
    see all

Images

  • Western blot - Anti-hUPF2/RENT2 antibody (ab157108)
    Western blot - Anti-hUPF2/RENT2 antibody (ab157108)
    All lanes : Anti-hUPF2/RENT2 antibody (ab157108) at 0.4 µg/ml

    Lane 1 : 293T whole cell lysate at 50 µg
    Lane 2 : 293T whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 148 kDa


    Exposure time: 30 seconds
  • Western blot - Anti-hUPF2/RENT2 antibody (ab157108)
    Western blot - Anti-hUPF2/RENT2 antibody (ab157108)
    All lanes : Anti-hUPF2/RENT2 antibody (ab157108) at 1 µg/ml

    Lane 1 : NIH 3T3 whole cell lysate
    Lane 2 : TCMK whole cell lysate
    Lane 3 : 4T1 whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 148 kDa


    Exposure time: 10 seconds
  • Immunoprecipitation - Anti-hUPF2/RENT2 antibody (ab157108)
    Immunoprecipitation - Anti-hUPF2/RENT2 antibody (ab157108)
    Detection of hUPF2/RENT2 in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab157108 at 6 µg/mg lysate for IP (Lane 1). For WB detection an ab157108 was used at 1 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.

Protocols

  • Western blot protocols
  • Immunoprecipitation protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab157108? Please let us know so that we can cite the reference in this datasheet.

ab157108 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Western blot abreview for Anti-hUPF2/RENT2 antibody

Poor
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing
Sample
Rat Cell lysate - whole cell (rat neonatal cardiomyocyte)
Specification
rat neonatal cardiomyocyte
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Read More

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Verified customer

Submitted Jul 16 2013

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