Western blot abreview for Anti-hUPF2/RENT2 antibody

Poor
Abreviews
Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing
Sample
Rat Cell lysate - whole cell (rat neonatal cardiomyocyte)
Specification
rat neonatal cardiomyocyte
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% BSA in TBS
Dilution
1/1000

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye­ 800CW
Dilution
1/5000

Detection

Detection method
fluorescence
Bands
Specific: none kDa Non-specific: 100, 60, 40, 25 kDa
Exposure
5 minute(s) and 0 second(s)
Negative control
siRNA knockdown of UPF2

Abcam user community

Verified customer

Submitted Jul 16 2013

Sign up