Overview

  • Product name

    Anti-hUPF2/RENT2 antibody - C-terminal
    See all hUPF2/RENT2 primary antibodies
  • Description

    Rabbit polyclonal to hUPF2/RENT2 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Xenopus laevis, Xenopus tropicalis
  • Immunogen

    Synthetic peptide corresponding to a region within C terminal amino acids 1200-1272 of Human hUPF2/RENT2.

  • Positive control

    • Mouse brain whole cell lysate; HeLa cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab153830 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 148 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Binds spliced mRNA.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Contains 3 MIF4G domains.
  • Cellular localization

    Cytoplasm > perinuclear region.
  • Information by UniProt
  • Database links

  • Alternative names

    • DKFZp434D222 antibody
    • hUpf2 antibody
    • KIAA1408 antibody
    • MGC138834 antibody
    • MGC138835 antibody
    • Nonsense mRNA reducing factor 2 antibody
    • Regulator of nonsense transcripts 2 antibody
    • RENT2 antibody
    • RENT2_HUMAN antibody
    • Smg 3 antibody
    • Smg 3 homolog nonsense mediated mRNA decay factor antibody
    • Up-frameshift suppressor 2 homolog antibody
    • UPF2 antibody
    • UPF2 regulator of nonsense transcripts homolog antibody
    • UROC1 antibody
    • Urocanate hydratase antibody
    • Yeast Upf2p homolog antibody
    see all

Images

  • Anti-hUPF2/RENT2 antibody - C-terminal (ab153830) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size: 148 kDa



    5% SDS PAGE
  • Immunofluorescent analysis of methanol-fixed HeLa cells labeling hUPF2/RENT2 with ab153830 at 1/500 dilution. Lower panel shows co-staining with Hoechst 33342.

References

This product has been referenced in:

  • Bao J  et al. UPF2-Dependent Nonsense-Mediated mRNA Decay Pathway Is Essential for Spermatogenesis by Selectively Eliminating Longer 3'UTR Transcripts. PLoS Genet 12:e1005863 (2016). Mouse . Read more (PubMed: 27149259) »
See 1 Publication for this product

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There are currently no Customer reviews or Questions for ab153830.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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