Key features and details
- Rabbit polyclonal to HURP
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-HURP antibody
See all HURP primary antibodies
DescriptionRabbit polyclonal to HURP
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Gorilla
Synthetic peptide corresponding to a region between residue 800 and the C-terminus (residue 846) of Human HURP (NP_055565.2).
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab70744 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 94 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionPotential cell cycle regulator that may play a role in carcinogenesis of cancer cells. Mitotic phosphoprotein regulated by the ubiquitin-proteasome pathway. Key regulator of adherens junction integrity and differentiation that may be involved in CDH1-mediated adhesion and signaling in epithelial cells.
Tissue specificityAbundantly expressed in fetal liver. Expressed at lower levels in bone marrow, testis, colon, and placenta.
Sequence similaritiesBelongs to the SAPAP family.
Developmental stageElevated levels of expression detected in the G2/M phase of synchronized cultures of HeLa cells.
modificationsUbiquitinated, leading to its degradation.
Phosphorylated upon DNA damage, probably by ATM or ATR. Decreased phosphorylation levels are associated with the differentiation of intestinal epithelial cells.
Cellular localizationNucleus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. Localizes to the spindle in mitotic cells. Colocalizes with CDH1 at sites of cell-cell contact in intestinal epithelial cells.
- Information by UniProt
- DAP-5 antibody
- DAP5 antibody
- Discs large homolog 7 antibody
Detection of HURP by Western Blot of Immunprecipitate. ab70744 at 1µg/ml staining HURP in HeLa whole cell lysate immunoprecipitated using ab70744 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Chemiluminescence with exposure time of 10 seconds.
All lanes : Anti-HURP antibody (ab70744) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 94 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
ab70744 has been referenced in 3 publications.
- Xiang Q et al. In vitro study of anti-ER positive breast cancer effect and mechanism of 1,2,3,4-6-pentyl-O-galloyl-beta-d-glucose (PGG). Biomed Pharmacother 111:813-820 (2019). PubMed: 30616080
- Zhang Y et al. The microtubule-associated protein HURP recruits the centrosomal protein TACC3 to regulate K-fiber formation and support chromosome congression. J Biol Chem 293:15733-15747 (2018). PubMed: 30054275
- Möckel MM et al. Xenopus laevis Kif18A is a highly processive kinesin required for meiotic spindle integrity. Biol Open 6:463-470 (2017). PubMed: 28228376