• Product name

  • Description

    Rabbit polyclonal to HURP
  • Host species

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: ICC/IF
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region between residues 200 and 250 of Human HURP (NP_055565.2).

  • Positive control

    • Human basal cell carcinoma and human seminoma tissues.



Our Abpromise guarantee covers the use of ab84509 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    IHC-P: 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining.
    WB: 1/200.

    Is unsuitable for ICC/IF.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Potential cell cycle regulator that may play a role in carcinogenesis of cancer cells. Mitotic phosphoprotein regulated by the ubiquitin-proteasome pathway. Key regulator of adherens junction integrity and differentiation that may be involved in CDH1-mediated adhesion and signaling in epithelial cells.
    • Tissue specificity

      Abundantly expressed in fetal liver. Expressed at lower levels in bone marrow, testis, colon, and placenta.
    • Sequence similarities

      Belongs to the SAPAP family.
    • Developmental stage

      Elevated levels of expression detected in the G2/M phase of synchronized cultures of HeLa cells.
    • Post-translational

      Ubiquitinated, leading to its degradation.
      Phosphorylated upon DNA damage, probably by ATM or ATR. Decreased phosphorylation levels are associated with the differentiation of intestinal epithelial cells.
    • Cellular localization

      Nucleus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. Localizes to the spindle in mitotic cells. Colocalizes with CDH1 at sites of cell-cell contact in intestinal epithelial cells.
    • Information by UniProt
    • Database links

    • Alternative names

      • DAP-5 antibody
      • DAP5 antibody
      • Discs large homolog 7 antibody
      • Disks large associated protein 5 antibody
      • Disks large associated protein DLG7 antibody
      • Disks large-associated protein 5 antibody
      • Disks large-associated protein DLG7 antibody
      • DLG 1 antibody
      • DLG 7 antibody
      • DLG1 antibody
      • DLG7 antibody
      • DLGAP5 antibody
      • DLGP5_HUMAN antibody
      • Drosophila discs large 1 tumor suppressor like antibody
      • Hepatoma up regulated protein antibody
      • Hepatoma up-regulated protein antibody
      • Hepatoma upregulated protein antibody
      • HURP antibody
      • KIAA0008 antibody
      see all


    • ab84509, at 1/250 dilution, staining HURP in formalin-fixed, paraffin-embedded human basal cell carcinoma by Immunohistochemistry, using DAB staining.
    • ab84509, at 1/250 dilution, staining HURP in formalin-fixed, paraffin-embedded human seminoma by Immunohistochemistry, using DAB staining.
    • Anti-HURP antibody (ab84509) at 1/200 dilution + 25µg of WCE from U20S cells synchronized by thimidine arrest and release to enrich in mitotic cells (same on lane 1 and 2).

      Image kindly provided by Patrizia Lavia, CNR National Research Council, University "La Sapienza", Rome, Italy.


    This product has been referenced in:

    • Zhang X  et al. Nucleolar and Spindle Associated Protein 1 (NUSAP1) Inhibits Cell Proliferation and Enhances Susceptibility to Epirubicin In Invasive Breast Cancer Cells by Regulating Cyclin D Kinase (CDK1) and DLGAP5 Expression. Med Sci Monit 24:8553-8564 (2018). Read more (PubMed: 30476929) »
    • Espinoza I  et al. Hypoxia on the Expression of Hepatoma Upregulated Protein in Prostate Cancer Cells. Front Oncol 6:144 (2016). IHC . Read more (PubMed: 27379206) »
    See all 3 Publications for this product

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