Key features and details
- Rabbit polyclonal to HURP
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-HURP antibody
See all HURP primary antibodies
DescriptionRabbit polyclonal to HURP
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to a region between residues 200 and 250 of Human HURP (NP_055565.2).
- Human basal cell carcinoma and human seminoma tissues.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab84509 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500.|
FunctionPotential cell cycle regulator that may play a role in carcinogenesis of cancer cells. Mitotic phosphoprotein regulated by the ubiquitin-proteasome pathway. Key regulator of adherens junction integrity and differentiation that may be involved in CDH1-mediated adhesion and signaling in epithelial cells.
Tissue specificityAbundantly expressed in fetal liver. Expressed at lower levels in bone marrow, testis, colon, and placenta.
Sequence similaritiesBelongs to the SAPAP family.
Developmental stageElevated levels of expression detected in the G2/M phase of synchronized cultures of HeLa cells.
modificationsUbiquitinated, leading to its degradation.
Phosphorylated upon DNA damage, probably by ATM or ATR. Decreased phosphorylation levels are associated with the differentiation of intestinal epithelial cells.
Cellular localizationNucleus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. Localizes to the spindle in mitotic cells. Colocalizes with CDH1 at sites of cell-cell contact in intestinal epithelial cells.
- Information by UniProt
- DAP-5 antibody
- DAP5 antibody
- Discs large homolog 7 antibody
ab84509, at 1/250 dilution, staining HURP in formalin-fixed, paraffin-embedded human basal cell carcinoma by Immunohistochemistry, using DAB staining.
ab84509, at 1/250 dilution, staining HURP in formalin-fixed, paraffin-embedded human seminoma by Immunohistochemistry, using DAB staining.
Anti-HURP antibody (ab84509) at 1/200 dilution + 25µg of WCE from U20S cells synchronized by thimidine arrest and release to enrich in mitotic cells (same on lane 1 and 2).
Image kindly provided by Patrizia Lavia, CNR National Research Council, University "La Sapienza", Rome, Italy.
ab84509 has been referenced in 3 publications.
- Zhang X et al. Nucleolar and Spindle Associated Protein 1 (NUSAP1) Inhibits Cell Proliferation and Enhances Susceptibility to Epirubicin In Invasive Breast Cancer Cells by Regulating Cyclin D Kinase (CDK1) and DLGAP5 Expression. Med Sci Monit 24:8553-8564 (2018). PubMed: 30476929
- Espinoza I et al. Hypoxia on the Expression of Hepatoma Upregulated Protein in Prostate Cancer Cells. Front Oncol 6:144 (2016). IHC . PubMed: 27379206
- Ma MP et al. Sorting nexin 9 recruits clathrin heavy chain to the mitotic spindle for chromosome alignment and segregation. PLoS One 8:e68387 (2013). PubMed: 23861900